biostudies-literatureShi ZScience and Technology Project of Tianjin Municipal Health Commissione70066https://www.ebi.ac.uk/biostudies/studies/S-EPMC12423771biostudies-literatureUnknown39(17)<h4>Background</h4>Advancements in non-small cell lung cancer (NSCLC) therapies have improved outcomes, but challenges like low immune response, drug resistance, and side effects persist. ACT001, a novel small-molecule inhibitor, shows promise in addressing these issues.<h4>Methods</h4>We evaluated ACT001's anti-tumor and immunomodulatory effects in NSCLC. In vitro, its impact on proliferation, migration, invasion, and cell cycle arrest was assessed. In vivo, its effect on tumor growth in C57BL/6 mice was studied. Pull-down assays and mass spectrometry identified ACT001's interaction with STAT1/STAT3 and its regulation of PD-L1 expression.<h4>Results</h4>ACT001 inhibited NSCLC cell proliferation, migration, and invasion, induced cell cycle arrest, and suppressed tumor growth in vivo. It enJournal of clinical laboratory analysisACT001 Inhibits Tumor Progression and Modulates Immune Responses in Non-Small Cell Lung Cancer.PMC12423771ZC20178Li YChen ZGuo HShi ZHou HWang ZYu YSong YfalseACT001 Inhibits Tumor Progression and Modulates Immune Responses in Non-Small Cell Lung Cancer.<h4>Background</h4>Advancements in non-small cell lung cancer (NSCLC) therapies have improved outcomes, but challenges like low immune response, drug resistance, and side effects persist. ACT001, a novel small-molecule inhibitor, shows promise in addressing these issues.<h4>Methods</h4>We evaluated ACT001's anti-tumor and immunomodulatory effects in NSCLC. In vitro, its impact on proliferation, migration, invasion, and cell cycle arrest was assessed. In vivo, its effect on tumor growth in C57BL/6 mice was studied. Pull-down assays and mass spectrometry identified ACT001's interaction with STAT1/STAT3 and its regulation of PD-L1 expression.<h4>Results</h4>ACT001 inhibited NSCLC cell proliferation, migration, and invasion, induced cell cycle arrest, and suppressed tumor growth in vivo. It en2025-01-01T00:00:00Z2025 Sep2026-06-01T23:33:04.707Z2026-05-24T03:07:08.816ZS-EPMC124237714083648910.1002/jcla.70066