{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Pollard KJ"],"funding":["NCATS NIH HHS","NINDS NIH HHS"],"pubmed_abstract":["Microphysiological systems (MPSs) are engineered, in vitro platforms which have been established as viable alternatives to animal models for pre-clinical research with unique advantages over conventional model systems. Many MPSs utilize 3-dimensional (3D) tissue constructs that enable biomimetic cell-cell interactions, allow for extended culture periods, and provide the time necessary for the emergence of physical and physiological characteristics of more mature tissues. Here, we present a novel MPS using human induced pluripotent stem cell (hiPSC)-derived spinal cord dorsal horn (SCDH) spheroids co-cultured with hiPSC-derived dorsal root ganglion (DRG) sensory spheroids in a microengineered hydrogel system to create a \"connectoid\" model of afferent pain circuitry. SCDH spheroids were func"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.08.31.673344"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12424732"],"repository":["biostudies-literature"],"pubmed_title":["Human microphysiological model of dorsal root ganglion-spinal cord dorsal horn circuitry recapitulates opioid induced effects."],"pmcid":["PMC12424732"],"funding_grant_id":["UH3 TR003150","F32 NS106740"],"pubmed_authors":["Moore MJ","Pollard KJ","Iyer NR","Ashton RS","Bosak A","Seipel FR"],"additional_accession":[]},"is_claimable":false,"name":"Human microphysiological model of dorsal root ganglion-spinal cord dorsal horn circuitry recapitulates opioid induced effects.","description":"Microphysiological systems (MPSs) are engineered, in vitro platforms which have been established as viable alternatives to animal models for pre-clinical research with unique advantages over conventional model systems. Many MPSs utilize 3-dimensional (3D) tissue constructs that enable biomimetic cell-cell interactions, allow for extended culture periods, and provide the time necessary for the emergence of physical and physiological characteristics of more mature tissues. Here, we present a novel MPS using human induced pluripotent stem cell (hiPSC)-derived spinal cord dorsal horn (SCDH) spheroids co-cultured with hiPSC-derived dorsal root ganglion (DRG) sensory spheroids in a microengineered hydrogel system to create a \"connectoid\" model of afferent pain circuitry. SCDH spheroids were func","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-04-27T03:13:47.658Z","creation":"2026-04-27T03:09:29.864Z"},"accession":"S-EPMC12424732","cross_references":{"pubmed":["40950113"],"doi":["10.1101/2025.08.31.673344"]}}