biostudies-literatureUnknownNewsom-Stewart CMNCI NIH HHSThe transcription factor SOX10 is a central regulator of melanoma biology, influencing tumor initiation, progression, phenotypic plasticity, and therapeutic resistance. However, the molecular mechanisms underlying these diverse functions remain incompletely understood. To elucidate the protein-protein interactions (PPIs) that mediate SOX10 activity in melanoma, we mapped the human SOX10 (hSOX10) interactome for the first time in human melanoma cells (A375 line). Given the challenges of capturing transient and weak transcription factor interactions, we employed miniTurbo (mT) proximity-dependent biotinylation coupled with mass spectrometry (MS). Stable A375 cell lines expressing N- and C-terminal mT-tagged hSOX10 fusion proteins at physiologically relevant levels were generated, enabling unbioRxiv : the preprint server for biology2025.09.03.672723https://www.ebi.ac.uk/biostudies/studies/S-EPMC12424987biostudies-literatureMapping of the hSOX10 protein interactome in human melanoma.PMC12424987R01 CA240633Newsom-Stewart CMBhatt DPMajor MBKaufman CKfalseMapping of the hSOX10 protein interactome in human melanoma.The transcription factor SOX10 is a central regulator of melanoma biology, influencing tumor initiation, progression, phenotypic plasticity, and therapeutic resistance. However, the molecular mechanisms underlying these diverse functions remain incompletely understood. To elucidate the protein-protein interactions (PPIs) that mediate SOX10 activity in melanoma, we mapped the human SOX10 (hSOX10) interactome for the first time in human melanoma cells (A375 line). Given the challenges of capturing transient and weak transcription factor interactions, we employed miniTurbo (mT) proximity-dependent biotinylation coupled with mass spectrometry (MS). Stable A375 cell lines expressing N- and C-terminal mT-tagged hSOX10 fusion proteins at physiologically relevant levels were generated, enabling un2025-01-01T00:00:00Z2025 Sep2026-04-29T03:18:58.997Z2026-04-29T03:13:03.188ZS-EPMC124249874095018110.1101/2025.09.03.672723