{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang JL"],"funding":["the Research Fund of Shanxi Province for Introduced High-level Leading Talents","the Special Research Fund of Shanxi Agricultural University for High-level Talents","National Natural Science Foundation of China","the Natural Science Foundation of Gansu Province, China","the Research Funding from the Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences","National Key Research and Development Program of China"],"pagination":["e1013475"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12425318"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(9)"],"pubmed_abstract":["Toxoplasma gondii is a significant pathogen in both humans and animals, with disease progression driven by the rapid proliferation of its tachyzoite stage. In this study, we identify the PP2A-2 holoenzyme as a key regulator of daughter cell emergence during parasite division. This holoenzyme, likely composed of the regulatory subunit TgPR48 (PP2A-B2), the catalytic subunit PP2A-C2, and the scaffolding subunit PP2A-A2, is essential for proper cytokinesis. Disruption of any single component severely impairs daughter cell separation and emergence. Phosphoproteomic analysis following PP2A-C2 depletion revealed numerous differentially phosphorylated proteins. Among these, DCS1 and DCS2 were prioritized as potential effectors. While phosphomimetic and non-phosphorylatable mutations in DCS1 and D"],"journal":["PLoS pathogens"],"pubmed_title":["The PP2A-2 holoenzyme orchestrates daughter cell emergence during cytokinesis in Toxoplasma gondii."],"pmcid":["PMC12425318"],"funding_grant_id":["2021XG001","2022YFD1800200 and 2022YFD1800201","RFSXIHLT202101","23JRRA1479 and 23JRRA555","CAAS-ASTIP-JBGS-20210801","23JRRA553","32422085"],"pubmed_authors":["Wang M","Fu BQ","Zhu XQ","Zhang NZ","Elsheikha HM","Gao J","Wu XJ","Li TT","Wang JL"],"additional_accession":[]},"is_claimable":false,"name":"The PP2A-2 holoenzyme orchestrates daughter cell emergence during cytokinesis in Toxoplasma gondii.","description":"Toxoplasma gondii is a significant pathogen in both humans and animals, with disease progression driven by the rapid proliferation of its tachyzoite stage. In this study, we identify the PP2A-2 holoenzyme as a key regulator of daughter cell emergence during parasite division. This holoenzyme, likely composed of the regulatory subunit TgPR48 (PP2A-B2), the catalytic subunit PP2A-C2, and the scaffolding subunit PP2A-A2, is essential for proper cytokinesis. Disruption of any single component severely impairs daughter cell separation and emergence. Phosphoproteomic analysis following PP2A-C2 depletion revealed numerous differentially phosphorylated proteins. Among these, DCS1 and DCS2 were prioritized as potential effectors. While phosphomimetic and non-phosphorylatable mutations in DCS1 and D","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-26T14:07:31.507Z","creation":"2026-05-24T03:07:08.695Z"},"accession":"S-EPMC12425318","cross_references":{"pubmed":["40901993"],"doi":["10.1371/journal.ppat.1013475"]}}