{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hagihara Y"],"funding":["NICHD NIH HHS","U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)"],"pagination":["2250-2263"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12425804"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["57(9)"],"pubmed_abstract":["Mammalian switch/sucrose nonfermentable (mSWI/SNF) complex regulates chromatin accessibility and frequently shows alterations due to mutation in cancer and neurological diseases. Inadequate expression of mSWI/SNF in heterozygous mice can lead to developmental defects, indicating dosage-sensitive effects of mSWI/SNF. However, how its dosage affects function has remained unclear. Using a targeted protein degradation system, we investigated its dosage-sensitive effects by precisely controlling protein levels of BRG1, the ATPase subunit of the mSWI/SNF complex. We found that binding of BRG1 to chromatin exhibited a linear response to the BRG1 protein level. Although chromatin accessibility at most promoters and insulators was largely unaffected by BRG1 depletion, 44% of enhancers, including 84"],"journal":["Nature genetics"],"pubmed_title":["Precise modulation of BRG1 levels reveals features of mSWI/SNF dosage sensitivity."],"pmcid":["PMC12425804"],"funding_grant_id":["R01HD092465","R01 HD092465"],"pubmed_authors":["Zhang Y","Zhang C","Hagihara Y"],"additional_accession":[]},"is_claimable":false,"name":"Precise modulation of BRG1 levels reveals features of mSWI/SNF dosage sensitivity.","description":"Mammalian switch/sucrose nonfermentable (mSWI/SNF) complex regulates chromatin accessibility and frequently shows alterations due to mutation in cancer and neurological diseases. Inadequate expression of mSWI/SNF in heterozygous mice can lead to developmental defects, indicating dosage-sensitive effects of mSWI/SNF. However, how its dosage affects function has remained unclear. Using a targeted protein degradation system, we investigated its dosage-sensitive effects by precisely controlling protein levels of BRG1, the ATPase subunit of the mSWI/SNF complex. We found that binding of BRG1 to chromatin exhibited a linear response to the BRG1 protein level. Although chromatin accessibility at most promoters and insulators was largely unaffected by BRG1 depletion, 44% of enhancers, including 84","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-01T12:05:37.749Z","creation":"2026-04-08T12:03:01.817Z"},"accession":"S-EPMC12425804","cross_references":{"pubmed":["40846763"],"doi":["10.1038/s41588-025-02305-z"]}}