{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shukla N"],"funding":["SGPGIMS","The German Federal Ministry of Education and Research (BMBF) project e: Med MelAutim","European Cooperation in Science and Technology - EU-Resolution Biology Network","Sanjay Gandhi Postgraduate Institute of Medical Sciences","Indian Council of Medical Research","Department of Science and Technology, Ministry of Science and Technology, India"],"pagination":["e70819"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12425813"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["29(17)"],"pubmed_abstract":["IL10 is a very effective anti-inflammatory cytokine. IL10 imbalance is linked to type 2 diabetes mellitus (T2DM) and also to renal hypertrophy, glomerular membrane thickening, and onset of diabetic nephropathy (DN). We aimed to investigate the association of IL10 gene polymorphism (rs1800871T/C, rs1800896A/G) with DN and determine the influence of variants on its expression level and interaction with transcription factors. We genotyped 301 study subjects, comprising 75 DN, 126 T2DM patients, and 100 controls. All were analysed for biochemical assays and genotypic analysis by PCR-RFLP and confirmed by Sanger sequencing. The haplotype analysis was calculated by Chi-square test. mRNA expression and its correlation with variants were assessed by using RT-PCR. Statistical analyses were done by "],"journal":["Journal of cellular and molecular medicine"],"pubmed_title":["Exploring the Prognostic Significance of IL10 Variants and Their Mechanistic Regulation in Diabetic Nephropathy."],"pmcid":["PMC12425813"],"funding_grant_id":["01ZX1905B","A-19-PGI/IMP/86/2022","A‐19‐PGI/IMP/86/2022","CA24138","3/1/3(18)/Endo‐fellowship/22‐NCD‐III","DST/INSPIRE/04/2015/001777","3/1/3(18)/Endo-fellowship/22-NCD-III"],"pubmed_authors":["Gupta S","Shukla N","Prasad N","Baden M","Verma P","Srivastava A","Janakiraman S","Rao GM","Wolkenhauer O","Gupta SK","Kumari S","Singh N","Gautam NK","Ansari MS"],"additional_accession":[]},"is_claimable":false,"name":"Exploring the Prognostic Significance of IL10 Variants and Their Mechanistic Regulation in Diabetic Nephropathy.","description":"IL10 is a very effective anti-inflammatory cytokine. IL10 imbalance is linked to type 2 diabetes mellitus (T2DM) and also to renal hypertrophy, glomerular membrane thickening, and onset of diabetic nephropathy (DN). We aimed to investigate the association of IL10 gene polymorphism (rs1800871T/C, rs1800896A/G) with DN and determine the influence of variants on its expression level and interaction with transcription factors. We genotyped 301 study subjects, comprising 75 DN, 126 T2DM patients, and 100 controls. All were analysed for biochemical assays and genotypic analysis by PCR-RFLP and confirmed by Sanger sequencing. The haplotype analysis was calculated by Chi-square test. mRNA expression and its correlation with variants were assessed by using RT-PCR. Statistical analyses were done by ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-01T12:04:33.19Z","creation":"2026-04-08T12:01:26.33Z"},"accession":"S-EPMC12425813","cross_references":{"pubmed":["40936203"],"doi":["10.1111/jcmm.70819"]}}