biostudies-literatureGomez-Lopez IMCIN/AEI/10.13039/501100011033 and "ERDF A way of making Europe"2934https://www.ebi.ac.uk/biostudies/studies/S-EPMC12427632biostudies-literatureUnknown14(17)<i>Opuntia stricta</i> var. <i>dillenii</i> (OPD) fruits are rich in betalains and phenolic compounds, which are recognized for their potential health-promoting properties. This study focuses on the optimization of pulsed electric field (PEF)-assisted solid-liquid green extraction (SLE) from OPD whole fruit, using response surface methodology (RSM) experimental design to obtain green extracts rich in bioactive compounds. The optimal PEF pre-treatment conditions (electric field strength and energy input) were determined based on the cell disintegration index (Zp), followed by optimizing SLE conditions (temperature, time, and ethanol content). High-performance liquid chromatography (HPLC-DAD-ESI-Qtof) was used to characterize the individual bioactive compound profile of the obtained OPD greeFoods (Basel, Switzerland)Pulsed Electric Field-Assisted "Green" Extraction of Betalains and Phenolic Compounds from <i>Opuntia stricta</i> var. <i>dillenii</i> Prickly Pears: Process Optimization and Biological Activity of Green Extracts.PMC12427632PID2020-118300RB-C21 and PID2020-118300RB-C22Ferrari GPirozzi APataro GPortillo MPCano MPCarpentieri SGomez-Lopez IfalsePulsed Electric Field-Assisted "Green" Extraction of Betalains and Phenolic Compounds from <i>Opuntia stricta</i> var. <i>dillenii</i> Prickly Pears: Process Optimization and Biological Activity of Green Extracts.<i>Opuntia stricta</i> var. <i>dillenii</i> (OPD) fruits are rich in betalains and phenolic compounds, which are recognized for their potential health-promoting properties. This study focuses on the optimization of pulsed electric field (PEF)-assisted solid-liquid green extraction (SLE) from OPD whole fruit, using response surface methodology (RSM) experimental design to obtain green extracts rich in bioactive compounds. The optimal PEF pre-treatment conditions (electric field strength and energy input) were determined based on the cell disintegration index (Zp), followed by optimizing SLE conditions (temperature, time, and ethanol content). High-performance liquid chromatography (HPLC-DAD-ESI-Qtof) was used to characterize the individual bioactive compound profile of the obtained OPD gree2025-01-01T00:00:00Z2025 Aug2026-04-08T19:10:47.475Z2026-04-08T12:02:29.168ZS-EPMC124276324094105010.3390/foods14172934