biostudies-literatureMa LWei Ma8131https://www.ebi.ac.uk/biostudies/studies/S-EPMC12427683biostudies-literatureUnknown26(17)Superoxide dismutase (SOD) is a key enzyme in the plant antioxidant system. It plays an essential role in plant adversity stress by scavenging excess reactive oxygen species to protect cells from oxidative damage. Isatis indigotica, being a mildly saline-tolerant plant, can be grown in soils containing a certain amount of saline-alkaline content. In order to reveal the SOD gene family members and their potential roles under saline and alkaline stress, the present study used a bioinformatics approach to identify 9 potential IiSOD genes in the I. indigotica genome. It analyzed the expression patterns of SOD family genes (IiSODs) in response to alkaline stress. According to the results of quantitative real-time PCR (qRT-PCR), the expression levels of the International journal of molecular sciencesGenome-Wide Expression Profile of SOD Gene Family in Isatis indigotica and the Key Role of IiSOD2 and IiSOD7 in Alkaline Stress.PMC12427683LJGXCG2023-058[2019] No. 5No.2021YFD1600901Wu JRen WKong LMa LZhang XJiang SMa JHe LWu WMa WfalseGenome-Wide Expression Profile of SOD Gene Family in Isatis indigotica and the Key Role of IiSOD2 and IiSOD7 in Alkaline Stress.Superoxide dismutase (SOD) is a key enzyme in the plant antioxidant system. It plays an essential role in plant adversity stress by scavenging excess reactive oxygen species to protect cells from oxidative damage. Isatis indigotica, being a mildly saline-tolerant plant, can be grown in soils containing a certain amount of saline-alkaline content. In order to reveal the SOD gene family members and their potential roles under saline and alkaline stress, the present study used a bioinformatics approach to identify 9 potential IiSOD genes in the I. indigotica genome. It analyzed the expression patterns of SOD family genes (IiSODs) in response to alkaline stress. According to the results of quantitative real-time PCR (qRT-PCR), the expression levels of the 2025-01-01T00:00:00Z2025 Aug2026-04-08T19:53:51.676Z2026-04-08T14:36:10.63ZS-EPMC124276834094305710.3390/ijms26178131