biostudies-literaturePenuela-Martinez AECenicaféGeneral Royalties System - Huila Government, in agreements signed with the National Colombian Coffee Growers Federation3001https://www.ebi.ac.uk/biostudies/studies/S-EPMC12427761biostudies-literatureUnknown14(17)Fermentation is a crucial stage in the production of washed mild coffees, as it enables the generation of compounds that influence overall quality. The conditions to optimize this process are still unknown. This study evaluated the effects of fermenting coffee fruits and depulped coffee under two conditions: an open tank (semi-anaerobic-SA) and a closed tank (self-induced anaerobic fermentation, SIAF) over 192 h. Samples were taken every 24 h using a sacrificial bioreactor. A randomized complete block design with a factorial arrangement (2 × 2 + 1), plus a standard control, was employed, incorporating two factors: coffee type and fermentation condition. High-throughput sequencing of 16S and ITS amplicons identified an average of 260 ± 71 and 101 ± 24 OTUs, respectively. <i>Weisella</i> wasFoods (Basel, Switzerland)Development of Fermentation Strategies for Quality Mild Coffee Production (&lt;i&gt;Coffea arabica&lt;/i&gt; L.) Based on Oxygen Availability and Processing Time.PMC1242776120221000100330Crossref Funder ID 100019597Penuela-Martinez AEBuitrago-Zuluaga CMedina-Rivera RDOsorio-Giraldo CVfalseDevelopment of Fermentation Strategies for Quality Mild Coffee Production (&lt;i&gt;Coffea arabica&lt;/i&gt; L.) Based on Oxygen Availability and Processing Time.Fermentation is a crucial stage in the production of washed mild coffees, as it enables the generation of compounds that influence overall quality. The conditions to optimize this process are still unknown. This study evaluated the effects of fermenting coffee fruits and depulped coffee under two conditions: an open tank (semi-anaerobic-SA) and a closed tank (self-induced anaerobic fermentation, SIAF) over 192 h. Samples were taken every 24 h using a sacrificial bioreactor. A randomized complete block design with a factorial arrangement (2 × 2 + 1), plus a standard control, was employed, incorporating two factors: coffee type and fermentation condition. High-throughput sequencing of 16S and ITS amplicons identified an average of 260 ± 71 and 101 ± 24 OTUs, respectively. <i>Weisella</i> was2025-01-01T00:00:00Z2025 Aug2026-05-26T03:08:05.881Z2026-05-26T03:06:37.315ZS-EPMC124277614094111710.3390/foods14173001