{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mariano A"],"funding":["Sapienza University of Rome"],"pagination":["8123"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12427997"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(17)"],"pubmed_abstract":["Intron retention (IR) is one of the cellular mechanisms to perform alternative splicing and thus control gene expression in several mammalian cellular pathways. IR in <i>PI-PLC γ1</i> mRNA was observed in some primary synoviocyte samples from osteoarthritis (OA) patients, likely due to inter-patient variability. The aim of the present manuscript was to explore the <i>PI-PLC γ1</i> IR molecular mechanism as a consequence of nutraceutical treatment of synoviocytes and the molecular basis of individual response. To evaluate the gene expression modulation of molecules involved in mRNA splicing, an RNA-seq analysis was performed, and the transcription modulation of six differentially expressed genes was validated by RT-PCR. Moreover, through a silencing experiment, the relationship between <i>P"],"journal":["International journal of molecular sciences"],"pubmed_title":["Molecular Basis of Intron Retention in &lt;i&gt;PI-PLC γ1&lt;/i&gt; mRNA from Osteoarthritis Synoviocytes."],"pmcid":["PMC12427997"],"funding_grant_id":["RP123188E2A80E21; AR2221814F245931"],"pubmed_authors":["Mattioli R","Ammendola S","Mariano A","D'Andrea D","Scotto d'Abusco A"],"additional_accession":[]},"is_claimable":false,"name":"Molecular Basis of Intron Retention in &lt;i&gt;PI-PLC γ1&lt;/i&gt; mRNA from Osteoarthritis Synoviocytes.","description":"Intron retention (IR) is one of the cellular mechanisms to perform alternative splicing and thus control gene expression in several mammalian cellular pathways. IR in <i>PI-PLC γ1</i> mRNA was observed in some primary synoviocyte samples from osteoarthritis (OA) patients, likely due to inter-patient variability. The aim of the present manuscript was to explore the <i>PI-PLC γ1</i> IR molecular mechanism as a consequence of nutraceutical treatment of synoviocytes and the molecular basis of individual response. To evaluate the gene expression modulation of molecules involved in mRNA splicing, an RNA-seq analysis was performed, and the transcription modulation of six differentially expressed genes was validated by RT-PCR. Moreover, through a silencing experiment, the relationship between <i>P","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-04-08T19:51:44.732Z","creation":"2026-04-08T14:35:29.421Z"},"accession":"S-EPMC12427997","cross_references":{"pubmed":["40943050"],"doi":["10.3390/ijms26178123"]}}