biostudies-literatureMariano ASapienza University of Rome8123https://www.ebi.ac.uk/biostudies/studies/S-EPMC12427997biostudies-literatureUnknown26(17)Intron retention (IR) is one of the cellular mechanisms to perform alternative splicing and thus control gene expression in several mammalian cellular pathways. IR in <i>PI-PLC γ1</i> mRNA was observed in some primary synoviocyte samples from osteoarthritis (OA) patients, likely due to inter-patient variability. The aim of the present manuscript was to explore the <i>PI-PLC γ1</i> IR molecular mechanism as a consequence of nutraceutical treatment of synoviocytes and the molecular basis of individual response. To evaluate the gene expression modulation of molecules involved in mRNA splicing, an RNA-seq analysis was performed, and the transcription modulation of six differentially expressed genes was validated by RT-PCR. Moreover, through a silencing experiment, the relationship between <i>PInternational journal of molecular sciencesMolecular Basis of Intron Retention in &lt;i&gt;PI-PLC γ1&lt;/i&gt; mRNA from Osteoarthritis Synoviocytes.PMC12427997RP123188E2A80E21; AR2221814F245931Mattioli RAmmendola SMariano AD'Andrea DScotto d'Abusco AfalseMolecular Basis of Intron Retention in &lt;i&gt;PI-PLC γ1&lt;/i&gt; mRNA from Osteoarthritis Synoviocytes.Intron retention (IR) is one of the cellular mechanisms to perform alternative splicing and thus control gene expression in several mammalian cellular pathways. IR in <i>PI-PLC γ1</i> mRNA was observed in some primary synoviocyte samples from osteoarthritis (OA) patients, likely due to inter-patient variability. The aim of the present manuscript was to explore the <i>PI-PLC γ1</i> IR molecular mechanism as a consequence of nutraceutical treatment of synoviocytes and the molecular basis of individual response. To evaluate the gene expression modulation of molecules involved in mRNA splicing, an RNA-seq analysis was performed, and the transcription modulation of six differentially expressed genes was validated by RT-PCR. Moreover, through a silencing experiment, the relationship between <i>P2025-01-01T00:00:00Z2025 Aug2026-04-08T19:51:44.732Z2026-04-08T14:35:29.421ZS-EPMC124279974094305010.3390/ijms26178123