{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Giugliano R"],"funding":["Italian Ministry of Health"],"pagination":["3031"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12428185"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(17)"],"pubmed_abstract":["Pistachio (<i>Pistacia vera</i>) is widely consumed among tree nuts but capable of triggering severe IgE-mediated reactions in allergic individuals. Due to the similarity of cashew-borne and pistachio-borne allergen proteins and DNA, traditional detection methods, such as ELISA and PCR, often suffer from cross-reactivity, limiting their ability to discriminate between these two allergens. This study presents a sensitive LC-MS/MS method for the simultaneous detection of pistachio and cashew allergens in processed food with a screening detection limit (SDL) equal to 1 mg/kg. The method was validated for specificity, SDL, β error, precision, and ruggedness, and applied to various matrices (cereals, chocolate, sauces, and meat products). Ruggedness testing showed that all considered parameters"],"journal":["Foods (Basel, Switzerland)"],"pubmed_title":["Allergens in Food: Analytical LC-MS/MS Method for the Qualitative Detection of &lt;i&gt;Pistacia vera&lt;/i&gt;."],"pmcid":["PMC12428185"],"funding_grant_id":["search code: IZSPLV 08/20 RC"],"pubmed_authors":["Lupi S","Bianchi DM","Morello S","Giugliano R","Vivaldi B","Razzuoli E"],"additional_accession":[]},"is_claimable":false,"name":"Allergens in Food: Analytical LC-MS/MS Method for the Qualitative Detection of &lt;i&gt;Pistacia vera&lt;/i&gt;.","description":"Pistachio (<i>Pistacia vera</i>) is widely consumed among tree nuts but capable of triggering severe IgE-mediated reactions in allergic individuals. Due to the similarity of cashew-borne and pistachio-borne allergen proteins and DNA, traditional detection methods, such as ELISA and PCR, often suffer from cross-reactivity, limiting their ability to discriminate between these two allergens. This study presents a sensitive LC-MS/MS method for the simultaneous detection of pistachio and cashew allergens in processed food with a screening detection limit (SDL) equal to 1 mg/kg. The method was validated for specificity, SDL, β error, precision, and ruggedness, and applied to various matrices (cereals, chocolate, sauces, and meat products). Ruggedness testing showed that all considered parameters","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-04-08T19:11:43.37Z","creation":"2026-04-08T12:03:25.469Z"},"accession":"S-EPMC12428185","cross_references":{"pubmed":["40941147"],"doi":["10.3390/foods14173031"]}}