biostudies-literatureGiugliano RItalian Ministry of Health3031https://www.ebi.ac.uk/biostudies/studies/S-EPMC12428185biostudies-literatureUnknown14(17)Pistachio (<i>Pistacia vera</i>) is widely consumed among tree nuts but capable of triggering severe IgE-mediated reactions in allergic individuals. Due to the similarity of cashew-borne and pistachio-borne allergen proteins and DNA, traditional detection methods, such as ELISA and PCR, often suffer from cross-reactivity, limiting their ability to discriminate between these two allergens. This study presents a sensitive LC-MS/MS method for the simultaneous detection of pistachio and cashew allergens in processed food with a screening detection limit (SDL) equal to 1 mg/kg. The method was validated for specificity, SDL, β error, precision, and ruggedness, and applied to various matrices (cereals, chocolate, sauces, and meat products). Ruggedness testing showed that all considered parametersFoods (Basel, Switzerland)Allergens in Food: Analytical LC-MS/MS Method for the Qualitative Detection of &lt;i&gt;Pistacia vera&lt;/i&gt;.PMC12428185search code: IZSPLV 08/20 RCLupi SBianchi DMMorello SGiugliano RVivaldi BRazzuoli EfalseAllergens in Food: Analytical LC-MS/MS Method for the Qualitative Detection of &lt;i&gt;Pistacia vera&lt;/i&gt;.Pistachio (<i>Pistacia vera</i>) is widely consumed among tree nuts but capable of triggering severe IgE-mediated reactions in allergic individuals. Due to the similarity of cashew-borne and pistachio-borne allergen proteins and DNA, traditional detection methods, such as ELISA and PCR, often suffer from cross-reactivity, limiting their ability to discriminate between these two allergens. This study presents a sensitive LC-MS/MS method for the simultaneous detection of pistachio and cashew allergens in processed food with a screening detection limit (SDL) equal to 1 mg/kg. The method was validated for specificity, SDL, β error, precision, and ruggedness, and applied to various matrices (cereals, chocolate, sauces, and meat products). Ruggedness testing showed that all considered parameters2025-01-01T00:00:00Z2025 Aug2026-04-08T19:11:43.37Z2026-04-08T12:03:25.469ZS-EPMC124281854094114710.3390/foods14173031