{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ren L"],"funding":["Lei Ren","Yangyan Wang","Yanyan Wang"],"pagination":["8141"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12428281"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(17)"],"pubmed_abstract":["Phthalic acid esters (PAEs), ubiquitously employed as a plasticizer, have been classified as priority environmental pollutants because of their persistence, bioaccumulation, and endocrine-disrupting properties. As a characterized PAE-degrading strain of marine origin, <i>Mycolicibacterium phocaicum</i> RL-HY01 utilizes di-(2-ethylhexyl) phthalate (DEHP) as its sole carbon and energy source. Genome sequencing and RT-qPCR analysis revealed a previously uncharacterized hydrolase gene (<i>dehpH</i>) in strain RL-HY01, which catalyzes ester bond cleavage in PAEs. Subsequently, recombinant expression of the cloned <i>dehpH</i> gene from strain RL-HY01 was established in <i>Escherichia coli</i> BL21(DE3). The purified recombinant DehpH exhibited optimal activity at 30 °C and pH 8.0. Its activity "],"journal":["International journal of molecular sciences"],"pubmed_title":["Identification and Characterization of a Novel Di-(2-ethylhexyl) Phthalate Hydrolase from a Marine Bacterial Strain &lt;i&gt;Mycolicibacterium phocaicum&lt;/i&gt; RL-HY01."],"pmcid":["PMC12428281"],"funding_grant_id":["College of Coastal Agricultural Sciences Distinguished Young Program (BH2025JCQN003)","Special Innovation Projects of Universities in Guangdong Province (2023KTSCX043)","Non-funded Projects of Zhanjiang City (2024B01096)","the National Natural Science Foundation of China(31800109 and 32271702)","Program for Scientific Research start-up Funds of Guangdong Ocean University (060302052312)","Guangdong Engineering Technology Research Center of Tropical Crops High-efficient Production (C16064)","Graduate Education Innovation Program of Guangdong Ocean University (202402)"],"pubmed_authors":["Shi M","Ren L","Zhou JL","Kuang C","Hu H","Xu D","Wang H","Wang Y"],"additional_accession":[]},"is_claimable":false,"name":"Identification and Characterization of a Novel Di-(2-ethylhexyl) Phthalate Hydrolase from a Marine Bacterial Strain &lt;i&gt;Mycolicibacterium phocaicum&lt;/i&gt; RL-HY01.","description":"Phthalic acid esters (PAEs), ubiquitously employed as a plasticizer, have been classified as priority environmental pollutants because of their persistence, bioaccumulation, and endocrine-disrupting properties. As a characterized PAE-degrading strain of marine origin, <i>Mycolicibacterium phocaicum</i> RL-HY01 utilizes di-(2-ethylhexyl) phthalate (DEHP) as its sole carbon and energy source. Genome sequencing and RT-qPCR analysis revealed a previously uncharacterized hydrolase gene (<i>dehpH</i>) in strain RL-HY01, which catalyzes ester bond cleavage in PAEs. Subsequently, recombinant expression of the cloned <i>dehpH</i> gene from strain RL-HY01 was established in <i>Escherichia coli</i> BL21(DE3). The purified recombinant DehpH exhibited optimal activity at 30 °C and pH 8.0. Its activity ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-04-08T18:35:16.821Z","creation":"2026-04-08T09:57:05.097Z"},"accession":"S-EPMC12428281","cross_references":{"pubmed":["40943065"],"doi":["10.3390/ijms26178141"]}}