biostudies-literatureParet CStiftung Kinderkrebsforschung MainzHI-TRON Mainz8431https://www.ebi.ac.uk/biostudies/studies/S-EPMC12428964biostudies-literatureUnknown26(17)High-risk (HR) neuroblastoma (NBL) patients often receive standardized treatment despite wide variations in clinical outcomes, underscoring the need for improved stratification tools. A distinguishing feature of NBL is the patient-specific expression of gangliosides (GGs), particularly GD2, which may serve as biomarkers. We analyzed GG profiles in 18 patient-derived tumors and 11 NBL cell lines using thin-layer chromatography and mass spectrometry. Expression of 0-, a-, and b-series GGs was examined and correlated with clinical risk, outcome, and gene expression data. Low-risk (LR) tumors expressed higher levels of complex b-series GGs. In HR tumors, five GG profiles (A-E) were identified. Profile A featured complex b-series GGs; B showed GD2 dominance; C showed synthesis arrest at GM3 or International journal of molecular sciencesGanglioside Profiling Uncovers Distinct Patterns in High-Risk Neuroblastoma.PMC12428964SKFM_01_2022HITR-2021-14Wingerter ABrignole CUstjanzew ASeidmann LSchwickerath PSandhoff RFaber JLudwig JEl Malki KPastorino FWagner SSathyamurthy SRoth WParet CfalseGanglioside Profiling Uncovers Distinct Patterns in High-Risk Neuroblastoma.High-risk (HR) neuroblastoma (NBL) patients often receive standardized treatment despite wide variations in clinical outcomes, underscoring the need for improved stratification tools. A distinguishing feature of NBL is the patient-specific expression of gangliosides (GGs), particularly GD2, which may serve as biomarkers. We analyzed GG profiles in 18 patient-derived tumors and 11 NBL cell lines using thin-layer chromatography and mass spectrometry. Expression of 0-, a-, and b-series GGs was examined and correlated with clinical risk, outcome, and gene expression data. Low-risk (LR) tumors expressed higher levels of complex b-series GGs. In HR tumors, five GG profiles (A-E) were identified. Profile A featured complex b-series GGs; B showed GD2 dominance; C showed synthesis arrest at GM3 or 2025-01-01T00:00:00Z2025 Aug2026-04-08T19:14:38.609Z2026-04-08T12:07:36.567ZS-EPMC124289644094335510.3390/ijms26178431