biostudies-literatureZhang XLiaodong University Doctoral Research Startup Fund ProjectLiaoning Provincial Joint Science and Technology Program (Key Technology Research and Development Project)8507https://www.ebi.ac.uk/biostudies/studies/S-EPMC12429396biostudies-literatureUnknown26(17)Nuclear Factor Y C (NF-YC) transcription factors (TFs) are central regulators of plant development and stress adaptation. However, there remains a gap in identifying NF-YC gene family members in blueberry (<i>Vaccinium corymbosum</i>), a globally significant fruit crop renowned for its nutritional value and good adaptability. In this study, a total of 31 <i>NF-YC</i> genes (designated <i>VcNF-YC1-31</i>) were identified in the blueberry genome, and their basic physicochemical properties, gene structures, motif patterns, and conserved domains were investigated using bioinformatic methods. The <i>cis</i>-acting elements in the promoters of <i>VcNF-YC</i>s were mainly enriched in phytohormone signaling, metabolism, and stress response. qRT-PCR analysis showed that <i>VcNF-YC</i>s were expressInternational journal of molecular sciencesGenome-Wide Identification and Expression of NF-YC Transcription Factors in Blueberry Under Abiotic Stress Conditions.PMC124293962024JH2/1026001312024BS052Huang JZhang XLiu BYu JXu XZhang BfalseGenome-Wide Identification and Expression of NF-YC Transcription Factors in Blueberry Under Abiotic Stress Conditions.Nuclear Factor Y C (NF-YC) transcription factors (TFs) are central regulators of plant development and stress adaptation. However, there remains a gap in identifying NF-YC gene family members in blueberry (<i>Vaccinium corymbosum</i>), a globally significant fruit crop renowned for its nutritional value and good adaptability. In this study, a total of 31 <i>NF-YC</i> genes (designated <i>VcNF-YC1-31</i>) were identified in the blueberry genome, and their basic physicochemical properties, gene structures, motif patterns, and conserved domains were investigated using bioinformatic methods. The <i>cis</i>-acting elements in the promoters of <i>VcNF-YC</i>s were mainly enriched in phytohormone signaling, metabolism, and stress response. qRT-PCR analysis showed that <i>VcNF-YC</i>s were express2025-01-01T00:00:00Z2025 Sep2026-05-26T17:02:27.071Z2026-05-26T03:06:07.879ZS-EPMC124293964094342810.3390/ijms26178507