biostudies-literatureZhang RRan ZhangXiaoxia Li2771https://www.ebi.ac.uk/biostudies/studies/S-EPMC12430373biostudies-literatureUnknown14(17)The tall wheatgrass <i>Thinopyrum ponticum</i> has excellent saline-alkali tolerance and great potential for restoring saline-alkali land to enhance productivity. This study used the <i>Thinopyrum ponticum</i> cv. "Orbit" variety, which is widely planted in saline-alkali pastures, as the material and artificially simulated salt stress using 150 mM NaCl and 150 mM Na₂SO₄, respectively. The growth and physiological indexes of the leaves and roots of seedlings were measured after various treatment durations, and the transcriptomes of untreated and Na₂SO₄-treated leaves and roots were also analyzed after 24 h of treatment. The results showed that salt stress resulted in significant reductions in leaf relative water content in seedlings and inhibited Plants (Basel, Switzerland)Physiological and Transcriptional Regulation of Salt Tolerance in &lt;i&gt;Thinopyrum ponticum&lt;/i&gt; and Screening of Salt-Tolerant Candidate Genes.PMC12430373project # CAFYBB2022XA002CCPTZX2023B01Liu YLi XZhong RNiu KJia FZhang RfalsePhysiological and Transcriptional Regulation of Salt Tolerance in &lt;i&gt;Thinopyrum ponticum&lt;/i&gt; and Screening of Salt-Tolerant Candidate Genes.The tall wheatgrass <i>Thinopyrum ponticum</i> has excellent saline-alkali tolerance and great potential for restoring saline-alkali land to enhance productivity. This study used the <i>Thinopyrum ponticum</i> cv. "Orbit" variety, which is widely planted in saline-alkali pastures, as the material and artificially simulated salt stress using 150 mM NaCl and 150 mM Na₂SO₄, respectively. The growth and physiological indexes of the leaves and roots of seedlings were measured after various treatment durations, and the transcriptomes of untreated and Na₂SO₄-treated leaves and roots were also analyzed after 24 h of treatment. The results showed that salt stress resulted in significant reductions in leaf relative water content in seedlings and inhibited 2025-01-01T00:00:00Z2025 Sep2026-04-08T19:13:01.145Z2026-04-08T12:05:30.971ZS-EPMC124303734094193510.3390/plants14172771