biostudies-literatureUnknown14(17)Yang YCotton fiber quality improvement remains a fundamental challenge in breeding programs due to the complex genetic architecture underlying fiber development. The narrow genetic base of upland cotton (<i>Gossypium hirsutum</i> L.) and the quantitative nature of fiber quality traits necessitate innovative approaches for identifying and incorporating superior alleles from related species. We developed a BC₆F₂ population by introgressing chromosome segments from the sea island cotton variety Xinhai 36 (<i>G. barbadense</i>) into the upland cotton variety Xinluzhong 60 (<i>G. hirsutum</i>). Based on fiber strength phenotyping, we constructed two DNA bulks representing extreme phenotypes (20 superior and 12 inferior individuals) for bulked segregant analysis sequencing (BSA-SPlants (Basel, Switzerland)2804https://www.ebi.ac.uk/biostudies/studies/S-EPMC12430576biostudies-literatureThe Genetic Loci Associated with Fiber Development in Upland Cotton (&lt;i&gt;Gossypium hirsutum&lt;/i&gt; L.) Were Mapped by the BSA-Seq Technique.PMC12430576Li YYang YZhang DLi CFu GSun FWei XMa JLai CWang ZfalseThe Genetic Loci Associated with Fiber Development in Upland Cotton (&lt;i&gt;Gossypium hirsutum&lt;/i&gt; L.) Were Mapped by the BSA-Seq Technique.Cotton fiber quality improvement remains a fundamental challenge in breeding programs due to the complex genetic architecture underlying fiber development. The narrow genetic base of upland cotton (<i>Gossypium hirsutum</i> L.) and the quantitative nature of fiber quality traits necessitate innovative approaches for identifying and incorporating superior alleles from related species. We developed a BC₆F₂ population by introgressing chromosome segments from the sea island cotton variety Xinhai 36 (<i>G. barbadense</i>) into the upland cotton variety Xinluzhong 60 (<i>G. hirsutum</i>). Based on fiber strength phenotyping, we constructed two DNA bulks representing extreme phenotypes (20 superior and 12 inferior individuals) for bulked segregant analysis sequencing (BSA-S2025-01-01T00:00:00Z2025 Sep2026-04-08T19:31:27.044Z2026-04-08T13:50:42.885ZS-EPMC124305764094196910.3390/plants14172804