{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Yevdash JI"],"funding":["NIDCR NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Melanocytes reside in diverse microenvironments that influence their susceptibility to oncogenic transformation, however, studying rare melanoma subsets has been hindered by the lack of suitable animal models. We developed a primary, immune-competent zebrafish model to study uveal melanoma (UM), utilizing choroidal-targeted injection and electroporation of plasmids containing human <i>GNAQ</i> <sup>Q209L</sup> and CRISPR/Cas9 cassettes for tumor suppressor gene deletion. Single-cell transcriptional profiling of genetically identical eye- and skin-derived tumors revealed distinct oncogenic pathways, highlighting the importance of studying melanoma subtypes in their correct anatomical context. Additionally, we identified a population of <i>tfec</i> - and <i>pax3a</i> -expressing melanocyte p"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.05.05.652300"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12431003"],"repository":["biostudies-literature"],"pubmed_title":["Mitfa-Independent Melanocyte Progenitors are Highly Susceptible to GNAQ-induced Uveal Melanoma in Adult Zebrafish."],"pmcid":["PMC12431003"],"funding_grant_id":["T90 DE023520","P30 CA086862","T32 GM144636"],"pubmed_authors":["Binkley EM","Cornell RA","Lang D","Moore R","Montgomery C","Friend D","Kenny C","O'Toole I","Yevdash JI","Weigel RJ","Gutelius D","Li Z","Riordan J","Dupuy A","Moore SPG","Robinson D","Campbell-Hanson KR"],"additional_accession":[]},"is_claimable":false,"name":"Mitfa-Independent Melanocyte Progenitors are Highly Susceptible to GNAQ-induced Uveal Melanoma in Adult Zebrafish.","description":"Melanocytes reside in diverse microenvironments that influence their susceptibility to oncogenic transformation, however, studying rare melanoma subsets has been hindered by the lack of suitable animal models. We developed a primary, immune-competent zebrafish model to study uveal melanoma (UM), utilizing choroidal-targeted injection and electroporation of plasmids containing human <i>GNAQ</i> <sup>Q209L</sup> and CRISPR/Cas9 cassettes for tumor suppressor gene deletion. Single-cell transcriptional profiling of genetically identical eye- and skin-derived tumors revealed distinct oncogenic pathways, highlighting the importance of studying melanoma subtypes in their correct anatomical context. Additionally, we identified a population of <i>tfec</i> - and <i>pax3a</i> -expressing melanocyte p","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-04-27T03:12:53.865Z","creation":"2026-04-27T03:09:25.639Z"},"accession":"S-EPMC12431003","cross_references":{"pubmed":["40950146"],"doi":["10.1101/2025.05.05.652300"]}}