biostudies-literatureUnknown62(10)Ponsuksili SThe cognitive and regulatory processes within higher-order brain structures that regulate the hypothalamic-pituitary-adrenal (HPA) axis and the limbic system orchestrate a complex stress response system. In order to address this, we collected 48 tissue samples from the amygdala (Amy), hippocampus (Hip), thalamus (Tal), hypothalamus (HT), pituitary gland (PG) and adrenal gland (AG). We applied ATAC-seq, a method for profiling accessible chromatin, to map the epigenetic landscape of these brain and endocrine tissues in pigs and generate foundational baseline chromatin accessibility datasets that can serve as a reference for future studies. A total of 321,584 consensus peaks, representing open chromatin regions across various samples and tissues in the pig genome, were identified. Screening fMolecular neurobiology13119-13135https://www.ebi.ac.uk/biostudies/studies/S-EPMC12433345biostudies-literatureTissue-Specific Chromatin Accessibility Regions and Transcription Factor Binding Sites in Pig Brain and Endocrine Tissues.PMC12433345Oster MHadlich FLi SPonsuksili STrakooljul NWimmers KReyer HfalseTissue-Specific Chromatin Accessibility Regions and Transcription Factor Binding Sites in Pig Brain and Endocrine Tissues.The cognitive and regulatory processes within higher-order brain structures that regulate the hypothalamic-pituitary-adrenal (HPA) axis and the limbic system orchestrate a complex stress response system. In order to address this, we collected 48 tissue samples from the amygdala (Amy), hippocampus (Hip), thalamus (Tal), hypothalamus (HT), pituitary gland (PG) and adrenal gland (AG). We applied ATAC-seq, a method for profiling accessible chromatin, to map the epigenetic landscape of these brain and endocrine tissues in pigs and generate foundational baseline chromatin accessibility datasets that can serve as a reference for future studies. A total of 321,584 consensus peaks, representing open chromatin regions across various samples and tissues in the pig genome, were identified. Screening f2025-01-01T00:00:00Z2025 Oct2026-04-08T18:40:11.278Z2026-04-08T10:07:31.061ZS-EPMC124333454048338510.1007/s12035-025-05128-5