biostudies-literatureLiu WLiaoning Province talent plan projectInnovative Teams Project in Key Areas of DalianDalian Medical Science Research Program256https://www.ebi.ac.uk/biostudies/studies/S-EPMC12433413biostudies-literatureUnknown151(9)<h4>Purpose</h4>The study aimed to investigate the role of FBXW11 in hepatocellular carcinoma (HCC) and its underlying mechanism. Specifically, we explored whether FBXW11 inhibits tumorigenesis by regulating YB1 ubiquitination and elucidated the functional significance of the FBXW11-YB1 axis in HCC progression.<h4>Methods</h4>Clinical HCC specimens and cell lines (HCC-LM3, HuH7, Hep3B, SNU-449) were used. FBXW11 and YB1 expression were analyzed via Western blotting and immunohistochemistry (IHC). Gain- and loss-of-function assays (FBXW11 overexpression/knockdown) were performed to assess cell proliferation. Co-immunoprecipitation (Co-IP), mass spectrometry, and ubiquitination assays identified protein interactions and ubiquitination patterns. In vivo tumorigenesis was evaluated using xenogJournal of cancer research and clinical oncologyFBXW11 inhibits tumorigenesis by ubiquitinating YB1 in hepatocarcinoma.PMC124334132021RT01YXMJ-JC-042312016Liu XXu BWang TDong CLiu WWang LfalseFBXW11 inhibits tumorigenesis by ubiquitinating YB1 in hepatocarcinoma.<h4>Purpose</h4>The study aimed to investigate the role of FBXW11 in hepatocellular carcinoma (HCC) and its underlying mechanism. Specifically, we explored whether FBXW11 inhibits tumorigenesis by regulating YB1 ubiquitination and elucidated the functional significance of the FBXW11-YB1 axis in HCC progression.<h4>Methods</h4>Clinical HCC specimens and cell lines (HCC-LM3, HuH7, Hep3B, SNU-449) were used. FBXW11 and YB1 expression were analyzed via Western blotting and immunohistochemistry (IHC). Gain- and loss-of-function assays (FBXW11 overexpression/knockdown) were performed to assess cell proliferation. Co-immunoprecipitation (Co-IP), mass spectrometry, and ubiquitination assays identified protein interactions and ubiquitination patterns. In vivo tumorigenesis was evaluated using xenog2025-01-01T00:00:00Z2025 Sep2026-04-08T19:52:07.522Z2026-04-08T14:35:43.283ZS-EPMC124334134094475710.1007/s00432-025-06307-6