{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tajer BJ"],"funding":["NICHD NIH HHS","NIGMS NIH HHS"],"pagination":["21-34"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12433595"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["240"],"pubmed_abstract":["The axolotl salamander model has broad utility for regeneration studies, but this model is limited by a lack of efficient cell-culture-based tools. The Axolotl Limb-1 (AL-1) fibroblast line, the only available immortalized axolotl cell line, was first published over 20 years ago, but many established molecular biology techniques, such as lipofectamine transfection, CRISPR-Cas9 mutagenesis, and antibiotic selection, work poorly or remain untested in AL-1 cells. Innovating technologies to manipulate AL-1 cells in culture and study their behavior following transplantation into the axolotl will complement in-vivo studies, decrease the number of animals used, and enable the faster, more streamlined investigation of regenerative biology questions. Here, we establish transfection, mutagenesis, an"],"journal":["Methods (San Diego, Calif.)"],"pubmed_title":["Optimized toolkit for the manipulation of immortalized axolotl fibroblasts."],"pmcid":["PMC12433595"],"funding_grant_id":["R25 GM109436","R01 HD095494"],"pubmed_authors":["Fei JF","Bohm S","Min S","Roy S","Souchet NR","Whited JL","Decaux A","Kalu G","Karabacak A","Sousounis K","Kim RT","Nelson JA","Blair SJ","Gilbert P","Lopez NJ","Courtemanche K","Froitzheim T","Harake N","Savage AM","Jay S","Wynn E","Kidd MD","Tanaka EM","Tajer BJ","Payzin-Dogru D","Han J","Luong AG","Singer HD"],"additional_accession":[]},"is_claimable":false,"name":"Optimized toolkit for the manipulation of immortalized axolotl fibroblasts.","description":"The axolotl salamander model has broad utility for regeneration studies, but this model is limited by a lack of efficient cell-culture-based tools. The Axolotl Limb-1 (AL-1) fibroblast line, the only available immortalized axolotl cell line, was first published over 20 years ago, but many established molecular biology techniques, such as lipofectamine transfection, CRISPR-Cas9 mutagenesis, and antibiotic selection, work poorly or remain untested in AL-1 cells. Innovating technologies to manipulate AL-1 cells in culture and study their behavior following transplantation into the axolotl will complement in-vivo studies, decrease the number of animals used, and enable the faster, more streamlined investigation of regenerative biology questions. Here, we establish transfection, mutagenesis, an","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-06-04T01:34:15.767Z","creation":"2026-05-04T03:12:22.598Z"},"accession":"S-EPMC12433595","cross_references":{"pubmed":["40187387"],"doi":["10.1016/j.ymeth.2025.03.019"]}}