biostudies-literatureUnknown16Jiang X<h4>Background</h4>Liver injury (LI) is responsible for a significant number of fatalities each year. In the context of Mongolian medicine, <i>Rhododendron molle</i> (Blume) G. Don (RM) is utilized for its properties to treatment of hepatic disorders. However, the underlying mechanisms of its action remain poorly understood.<h4>Objectives</h4>Clarifying the process through which RM enhances LI.<h4>Methods</h4>The chemical constituents were subjected to analysis, and network pharmacology alongside molecular docking studies were conducted. Additionally, ELISA, staining techniques, metabolomic analyses, and 16S rDNA sequencing were performed.<h4>Results</h4>A total of 17 components have been identified from RM, including liver disease-related compounds such as kaempferol, emodin, quercetin. NFrontiers in microbiology1570229https://www.ebi.ac.uk/biostudies/studies/S-EPMC12434124biostudies-literatureIntegrative network pharmacology, metabolomics and gut flora studies reveal mechanisms of action of &lt;i&gt;Rhododendron molle&lt;/i&gt; (Blume) G. Don to ameliorate liver injury.PMC12434124Li XMeng THe DJiang XMeng HChang HZhuang YfalseIntegrative network pharmacology, metabolomics and gut flora studies reveal mechanisms of action of &lt;i&gt;Rhododendron molle&lt;/i&gt; (Blume) G. Don to ameliorate liver injury.<h4>Background</h4>Liver injury (LI) is responsible for a significant number of fatalities each year. In the context of Mongolian medicine, <i>Rhododendron molle</i> (Blume) G. Don (RM) is utilized for its properties to treatment of hepatic disorders. However, the underlying mechanisms of its action remain poorly understood.<h4>Objectives</h4>Clarifying the process through which RM enhances LI.<h4>Methods</h4>The chemical constituents were subjected to analysis, and network pharmacology alongside molecular docking studies were conducted. Additionally, ELISA, staining techniques, metabolomic analyses, and 16S rDNA sequencing were performed.<h4>Results</h4>A total of 17 components have been identified from RM, including liver disease-related compounds such as kaempferol, emodin, quercetin. N2025-01-01T00:00:00Z20252026-06-02T05:55:09.125Z2026-04-15T03:09:35.228ZS-EPMC124341244095922110.3389/fmicb.2025.1570229