{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(12)"],"submitter":["Yang X"],"pubmed_abstract":["Spermidine metabolism influences tumor progression and anti-tumor immunity, thereby affecting treatment sensitivity. However, the precise role and therapeutic potential of spermidine in breast cancer remain unclear. Integrated multi-omics analyses (bulk and single-cell RNA sequencing) revealed a significant positive correlation between intratumoral spermidine abundance and immunophenotypic markers of CD8<sup>+</sup> T cell infiltration and activation (GZMB<sup>+</sup>CD8<sup>+</sup> T cells). Immunohistochemical and multiplexed immunohistochemistry validation (IHC/mIHC) demonstrated that breast cancer specimens with elevated spermidine production exhibited increased numbers of activated CD8⁺ T cells. Exogenous supplementation with spermidine promoted CD8⁺ T cell activation directly. Furthermore, supplementing spermidine <i>in vivo</i> promoted anti-tumor immune responses and enhanced sensitivity to anti-PD-1 immunotherapy combined with chemotherapy. Our findings indicate that boosting spermidine metabolism is a promising strategy to reinvigorate CD8⁺ T cell function and improve the efficacy of checkpoint blockade immunotherapy."],"journal":["Journal of Cancer"],"pagination":["3684-3695"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12435280"],"repository":["biostudies-literature"],"pubmed_title":["Spermidine potentiates anti-tumor immune responses and immunotherapy sensitivity in breast cancer."],"pmcid":["PMC12435280"],"pubmed_authors":["Yang X","Gao B","Wang R","Feng Y","Zeng X","Chen H","Zeng W","Huang P"],"additional_accession":[]},"is_claimable":false,"name":"Spermidine potentiates anti-tumor immune responses and immunotherapy sensitivity in breast cancer.","description":"Spermidine metabolism influences tumor progression and anti-tumor immunity, thereby affecting treatment sensitivity. However, the precise role and therapeutic potential of spermidine in breast cancer remain unclear. Integrated multi-omics analyses (bulk and single-cell RNA sequencing) revealed a significant positive correlation between intratumoral spermidine abundance and immunophenotypic markers of CD8<sup>+</sup> T cell infiltration and activation (GZMB<sup>+</sup>CD8<sup>+</sup> T cells). Immunohistochemical and multiplexed immunohistochemistry validation (IHC/mIHC) demonstrated that breast cancer specimens with elevated spermidine production exhibited increased numbers of activated CD8⁺ T cells. Exogenous supplementation with spermidine promoted CD8⁺ T cell activation directly. Furthermore, supplementing spermidine <i>in vivo</i> promoted anti-tumor immune responses and enhanced sensitivity to anti-PD-1 immunotherapy combined with chemotherapy. Our findings indicate that boosting spermidine metabolism is a promising strategy to reinvigorate CD8⁺ T cell function and improve the efficacy of checkpoint blockade immunotherapy.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-06-02T05:23:32.103Z","creation":"2026-04-14T03:13:57.199Z"},"accession":"S-EPMC12435280","cross_references":{"pubmed":["40959110"],"doi":["10.7150/jca.113235"]}}