biostudies-literatureUnknown21(12)Xu Q<b>Background</b>: <i>N</i> ⁶-methyladenosine (m⁶A) modification plays a crucial role in various physiological processes by regulating mRNA biology. However, the exact impact of m⁶A modification on macrophages in adipose tissues under obese settings remains to be further elucidated. <b>Methods</b>: We established macrophage-specific <i>Wtap</i>-deficient mice to explore the effects of <i>Wtap</i> on obesity and metabolic disorders induced by high-fat diet (HFD) in mice. The molecular targets were explored by MeRIP-qPCR, and the metabolomic assays were performed to detect the alteration of relevant metabolites. <b>Results</b>: Wilms tumor 1-associated protein (WTAP), one of the m⁶A "writers", was downregulated in adipose tissue macrophages (ATMs) International journal of biological sciences5428-5443https://www.ebi.ac.uk/biostudies/studies/S-EPMC12435483biostudies-literatureWTAP Maintains Alternative Activation of Macrophages by Promoting IDH1-Mediated α-ketoglutarate Production.PMC12435483Liu SWZhang JGong QXu QChen LYu QZhang SZou YLi YWang CYWang TYang PSun FLuo XXiong FfalseWTAP Maintains Alternative Activation of Macrophages by Promoting IDH1-Mediated α-ketoglutarate Production.<b>Background</b>: <i>N</i> ⁶-methyladenosine (m⁶A) modification plays a crucial role in various physiological processes by regulating mRNA biology. However, the exact impact of m⁶A modification on macrophages in adipose tissues under obese settings remains to be further elucidated. <b>Methods</b>: We established macrophage-specific <i>Wtap</i>-deficient mice to explore the effects of <i>Wtap</i> on obesity and metabolic disorders induced by high-fat diet (HFD) in mice. The molecular targets were explored by MeRIP-qPCR, and the metabolomic assays were performed to detect the alteration of relevant metabolites. <b>Results</b>: Wilms tumor 1-associated protein (WTAP), one of the m⁶A "writers", was downregulated in adipose tissue macrophages (ATMs) 2025-01-01T00:00:00Z20252026-06-03T02:56:36.491Z2026-04-23T03:13:35.508ZS-EPMC124354834095928310.7150/ijbs.115672