{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["De La Cruz AC"],"funding":["HHS | National Institutes of Health","HHS | National Institutes of Health (NIH)","Howard Hughes Medical Institute","NSF | National Science Foundation Graduate Research Fellowship Program","NINDS NIH HHS","National Science Foundation (NSF)","NSF | National Science Foundation Graduate Research Fellowship Program (GRFP)","NIGMS NIH HHS","National Science Foundation"],"pagination":["5215-5232"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12436648"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["44(18)"],"pubmed_abstract":["Ribosome-associated protein quality control (RQC) is a surveillance system that identifies and processes aberrant mRNAs with collided ribosomes. ZNF598 plays a key role by ubiquitinating the 40S subunit of collided ribosomes. However, how ZNF598 distinguishes stalled from transient ribosome collisions remains unclear. To address this, we developed a method to visualize the binding of a single protein to a specific mRNA while simultaneously determining its translation status. By endogenously tagging ZNF598 with HaloTag, we observed its strong interaction with RQC reporter mRNAs. We discovered that multiple ZNF598s engage with a single RQC mRNA, suggesting that ZNF598 recognizes more than just the leading collided ribosome in a queue. Overexpressing ZNF598 increased the ribosomal clearance r"],"journal":["The EMBO journal"],"pubmed_title":["Single-protein/RNA imaging reveals ZNF598 as a limiting factor in resolving collided ribosomes."],"pmcid":["PMC12436648"],"funding_grant_id":["MCB1817447","T32 GM135131","T32 GM007445","DGE1746891","R01GM138770","T32-GM135131","T32-GM007445","RF1NS113820","RF1 NS113820"],"pubmed_authors":["Tisdale G","Green R","Huang Z","Nakayama E","Sinha NK","Wu B","De La Cruz AC"],"additional_accession":[]},"is_claimable":false,"name":"Single-protein/RNA imaging reveals ZNF598 as a limiting factor in resolving collided ribosomes.","description":"Ribosome-associated protein quality control (RQC) is a surveillance system that identifies and processes aberrant mRNAs with collided ribosomes. ZNF598 plays a key role by ubiquitinating the 40S subunit of collided ribosomes. However, how ZNF598 distinguishes stalled from transient ribosome collisions remains unclear. To address this, we developed a method to visualize the binding of a single protein to a specific mRNA while simultaneously determining its translation status. By endogenously tagging ZNF598 with HaloTag, we observed its strong interaction with RQC reporter mRNAs. We discovered that multiple ZNF598s engage with a single RQC mRNA, suggesting that ZNF598 recognizes more than just the leading collided ribosome in a queue. Overexpressing ZNF598 increased the ribosomal clearance r","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-04-08T19:55:04.199Z","creation":"2026-04-08T14:36:22.346Z"},"accession":"S-EPMC12436648","cross_references":{"pubmed":["40750700"],"doi":["10.1038/s44318-025-00523-z"]}}