biostudies-literatureUnknown71(4)Handoh T<h4>Objectives</h4>Dilated cardiomyopathy (DCM) is one of the leading causes of heart failure. To date, 48 genes are known to be associated with DCM. Telethonin, encoded by the <i>TCAP</i> gene, is a Z-disk protein that composes cytoskeletal structures and facilitates various signaling pathways in cardiomyocytes. At least, six <i>TCAP</i> variants have been found in patients with DCM. We sought to investigate the role of <i>TCAP</i> in cardiac function using <i>TCAP</i>-knockdown (KD) iPS cell (iPSC)-induced cardiomyocytes (CMs).<h4>Methods & results</h4>To investigate the role of <i>TCAP</i> in cardiomyocytes, the <i>TCAP</i> gene was knocked down in human iPS cells established from a healthy subject (201B7) using the CRISPR-Cas9 genome editing. The expressions of <i>TCAP</i> mRNA and telJuntendo medical journal247-255https://www.ebi.ac.uk/biostudies/studies/S-EPMC12441175biostudies-literatureKnockdown of Telethonin Reduces Contractions and Provokes Aberrant Ca&lt;sup&gt;2+&lt;/sup&gt;-waves in Human iPS Cell-induced Cardiomyocytes.PMC12441175Hori AAi TInaba HYamamoto HHoriuchi YTabe YHandoh TTurker IMiida TfalseKnockdown of Telethonin Reduces Contractions and Provokes Aberrant Ca&lt;sup&gt;2+&lt;/sup&gt;-waves in Human iPS Cell-induced Cardiomyocytes.<h4>Objectives</h4>Dilated cardiomyopathy (DCM) is one of the leading causes of heart failure. To date, 48 genes are known to be associated with DCM. Telethonin, encoded by the <i>TCAP</i> gene, is a Z-disk protein that composes cytoskeletal structures and facilitates various signaling pathways in cardiomyocytes. At least, six <i>TCAP</i> variants have been found in patients with DCM. We sought to investigate the role of <i>TCAP</i> in cardiac function using <i>TCAP</i>-knockdown (KD) iPS cell (iPSC)-induced cardiomyocytes (CMs).<h4>Methods & results</h4>To investigate the role of <i>TCAP</i> in cardiomyocytes, the <i>TCAP</i> gene was knocked down in human iPS cells established from a healthy subject (201B7) using the CRISPR-Cas9 genome editing. The expressions of <i>TCAP</i> mRNA and tel2025-01-01T00:00:00Z20252026-04-27T03:17:14.125Z2026-04-27T03:11:10.475ZS-EPMC124411754096982210.14789/ejmj.JMJ24-0025-OA