biostudies-literatureUnknown54(3)DiCiccio VK<h4>Background</h4>A pre-transfusion blood crossmatch is recommended to ensure RBC compatibility for previously transfused and transfusion-naïve cats.<h4>Objectives</h4>To compare 3 crossmatch methods and anti-feline globulin (AFG) enhancement for determining RBC compatibility.<h4>Methods</h4>Prospective study. Major crossmatches were performed using serum from 68 "recipient" cats and RBCs from 44 "donor" cats. Crossmatch methods evaluated include laboratory tube and gel column without and with AFG enhancement and an in-clinic AFG-enhanced immunochromatographic strip.<h4>Results</h4>Tube and gel crossmatches were performed for 211 and strip crossmatches for 133 recipient-donor pairs. RBC incompatibilities were noted in at least 1 crossmatch method for 123 recipient-donor pairs. For determiVeterinary clinical pathology230-238https://www.ebi.ac.uk/biostudies/studies/S-EPMC12444005biostudies-literatureComparison of Three Feline Crossmatch Methods-Tube, Gel Column, and Immunochromatographic Strip.PMC12444005DiCiccio VKHess RSWeinstein NMFromm SCallan MBMarryott KGonzalez RfalseComparison of Three Feline Crossmatch Methods-Tube, Gel Column, and Immunochromatographic Strip.<h4>Background</h4>A pre-transfusion blood crossmatch is recommended to ensure RBC compatibility for previously transfused and transfusion-naïve cats.<h4>Objectives</h4>To compare 3 crossmatch methods and anti-feline globulin (AFG) enhancement for determining RBC compatibility.<h4>Methods</h4>Prospective study. Major crossmatches were performed using serum from 68 "recipient" cats and RBCs from 44 "donor" cats. Crossmatch methods evaluated include laboratory tube and gel column without and with AFG enhancement and an in-clinic AFG-enhanced immunochromatographic strip.<h4>Results</h4>Tube and gel crossmatches were performed for 211 and strip crossmatches for 133 recipient-donor pairs. RBC incompatibilities were noted in at least 1 crossmatch method for 123 recipient-donor pairs. For determi2025-01-01T00:00:00Z2025 Sep2026-06-03T11:26:13.338Z2026-04-27T03:10:30.299ZS-EPMC124440054084346710.1111/vcp.70039