{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["O'Brien CA"],"funding":["National Institute of Neurological Disorders and Stroke","Children's Hospital of Philadelphia","Silicon Valley Community Foundation","NINDS NIH HHS","National Institute of Dental and Craniofacial Research","National Institutes of Health","NIH HHS"],"pagination":["6248-6264"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12444772"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33(12)"],"pubmed_abstract":["Microglia replacement therapy, where endogenous brain macrophages are depleted and replaced by adoptively transferred surrogates, holds promise for treating pediatric neurologic diseases, but little is known about how early-life microglia replacement impacts the brain. We sought to investigate how early postnatal microglia depletion and adoptive macrophage transfer, essential components of microglia replacement, durably impact neural circuits in a mouse model. Using both pharmacologic and genetic models, postnatal microglia depletion worsened adult seizure severity, mortality, and neuropathology in a chemical seizure model. Replacement of endogenous microglia by adoptive transfer of monocytes rescued this effect, while transfer of authentic microglia from a donor mouse did not, and even wo"],"journal":["Molecular therapy : the journal of the American Society of Gene Therapy"],"pubmed_title":["Neonatal microglia replacement in mice modulates seizure severity in adulthood."],"pmcid":["PMC12444772"],"funding_grant_id":["R21 NS135366","DP5 OD036159","DP5OD036159","R21 NS128745","T32 NS007413","R01 NS120099","2023-33184","R01 NS129737","R01 NS120960","R01-NS-120960","R01-NS-129737-01"],"pubmed_authors":["Cohen AS","Bennett ML","Bennett FC","Shukla S","Levitt EM","Delcy SAS","O'Brien CA"],"additional_accession":[]},"is_claimable":false,"name":"Neonatal microglia replacement in mice modulates seizure severity in adulthood.","description":"Microglia replacement therapy, where endogenous brain macrophages are depleted and replaced by adoptively transferred surrogates, holds promise for treating pediatric neurologic diseases, but little is known about how early-life microglia replacement impacts the brain. We sought to investigate how early postnatal microglia depletion and adoptive macrophage transfer, essential components of microglia replacement, durably impact neural circuits in a mouse model. Using both pharmacologic and genetic models, postnatal microglia depletion worsened adult seizure severity, mortality, and neuropathology in a chemical seizure model. Replacement of endogenous microglia by adoptive transfer of monocytes rescued this effect, while transfer of authentic microglia from a donor mouse did not, and even wo","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-07-15T07:45:38.084Z","creation":"2026-07-01T03:08:03.588Z"},"accession":"S-EPMC12444772","cross_references":{"pubmed":["40873033"],"doi":["10.1016/j.ymthe.2025.08.039"]}}