biostudies-literatureMandal JNIDDK NIH HHSNHLBI NIH HHS1729-1742https://www.ebi.ac.uk/biostudies/studies/S-EPMC12444880biostudies-literatureUnknown82(10)<h4>Background</h4>Previously, we demonstrated that the ketone body, β-hydroxybutyrate, is a potent antihypertensive and reno-protective metabolite in Dahl Salt-Sensitive rats. However, the mechanism by which β-hydroxybutyrate confers these beneficial effects is understudied. Here we focused on determining whether the reno-protective effect of β-hydroxybutyrate is due to its known ability to epigenetically remodel chromatin via histone β-hydroxybutyrylation.<h4>Methods</h4>We used the same animal protocol previously used for the discovery of the reno-protective effect of β-hydroxybutyrate. Briefly, postweaning, male and female Dahl Salt-Sensitive rats were split into 2 groups and supplemented with or without 1,3-butanediol for 6 weeks. At euthanasia, circulating β-hydroxybutyrate was quantHypertension (Dallas, Tex. : 1979)Epigenetic Histone β-Hydroxybutyrylation Contributes to Renoprotection by β-Hydroxybutyrate in the Dahl Rat.PMC12444880R01 HL171401R01 DK134053R01 HL143082Joe BMandal JMei XTummala RKleinhenz AAryal Sde la Serna IManandhar IBasrur VGunning WTChakraborty SYeoh BSYang TMell BSaha PVijay-Kumar MfalseEpigenetic Histone β-Hydroxybutyrylation Contributes to Renoprotection by β-Hydroxybutyrate in the Dahl Rat.<h4>Background</h4>Previously, we demonstrated that the ketone body, β-hydroxybutyrate, is a potent antihypertensive and reno-protective metabolite in Dahl Salt-Sensitive rats. However, the mechanism by which β-hydroxybutyrate confers these beneficial effects is understudied. Here we focused on determining whether the reno-protective effect of β-hydroxybutyrate is due to its known ability to epigenetically remodel chromatin via histone β-hydroxybutyrylation.<h4>Methods</h4>We used the same animal protocol previously used for the discovery of the reno-protective effect of β-hydroxybutyrate. Briefly, postweaning, male and female Dahl Salt-Sensitive rats were split into 2 groups and supplemented with or without 1,3-butanediol for 6 weeks. At euthanasia, circulating β-hydroxybutyrate was quant2025-01-01T00:00:00Z2025 Oct2026-07-16T18:42:34.873Z2026-07-11T03:08:21.629ZS-EPMC124448804072639310.1161/HYPERTENSIONAHA.125.25251