{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Harle V"],"funding":["Health Research Charities Ireland","OpenTargets","Astra Zeneca","Wellcome Trust"],"pagination":["284"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12445041"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(1)"],"pubmed_abstract":["<h4>Background</h4>Synthetic lethal interactions are attractive therapeutic candidates as they enable selective targeting of cancer cells in which somatic alterations have disrupted one member of a synthetic lethal gene pair while leaving normal tissues untouched, thus minimising off-target toxicity. Despite this potential, the number of well-established and validated synthetic lethal gene pairs is modest.<h4>Results</h4>We generate a dual-guide CRISPR/Cas9 Library and analyse 472 predicted synthetic lethal pairs in 27 cancer cell Lines from melanoma, pancreatic and lung cancer Lineages. We report a robust collection of 117 genetic interactions within and across cancer types and explore their candidacy as therapeutic targets. We show that SLC25A28 is an attractive target since its syntheti"],"journal":["Genome biology"],"pubmed_title":["A compendium of synthetic lethal gene pairs defined by extensive combinatorial pan-cancer CRISPR screening."],"pmcid":["PMC12445041"],"funding_grant_id":["220540/Z/20/A","20/FFP-P/8641 and 18/CRT/6214"],"pubmed_authors":["Olvera-Leon R","Kebabci N","Thompson NA","Li L","Mehta I","Offord V","Ryan CJ","Peidli S","van de Haar J","Adams DJ","Alexander D","Goncalves E","Harle V","Gokbag B","De Kegel B","Williams T","Iyer V","Fotopoulos L"],"additional_accession":[]},"is_claimable":false,"name":"A compendium of synthetic lethal gene pairs defined by extensive combinatorial pan-cancer CRISPR screening.","description":"<h4>Background</h4>Synthetic lethal interactions are attractive therapeutic candidates as they enable selective targeting of cancer cells in which somatic alterations have disrupted one member of a synthetic lethal gene pair while leaving normal tissues untouched, thus minimising off-target toxicity. Despite this potential, the number of well-established and validated synthetic lethal gene pairs is modest.<h4>Results</h4>We generate a dual-guide CRISPR/Cas9 Library and analyse 472 predicted synthetic lethal pairs in 27 cancer cell Lines from melanoma, pancreatic and lung cancer Lineages. We report a robust collection of 117 genetic interactions within and across cancer types and explore their candidacy as therapeutic targets. We show that SLC25A28 is an attractive target since its syntheti","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T15:40:13.295Z","creation":"2026-04-29T03:12:40.215Z"},"accession":"S-EPMC12445041","cross_references":{"pubmed":["40968372"],"doi":["10.1186/s13059-025-03737-w"]}}