biostudies-literatureZou ZResearch Cooperation Platform Project of Sino-Japanese Friendship Hospital of Jilin University and Basic Medical School of Jilin UniversityNational Natural Science Foundation of ChinaDepartment of Science and Technology of Jilin Provincee70615https://www.ebi.ac.uk/biostudies/studies/S-EPMC12445200biostudies-literatureUnknown31(9)<h4>Introduction</h4>Ferroptosis is an essential pathophysiological process in cerebral ischemic injury. Activin receptor-interacting protein 1 (ARIP1) is a negative regulator of the activin signaling pathway in neurons.<h4>Objective</h4>This study investigated whether activin A inhibits neuronal ferroptosis and the role of ARIP1 in cerebral ischemic injury.<h4>Methods and results</h4>In this study, activin A increased the viability of primary neurons under conditions of oxygen-glucose deprivation (OGD). Subsequent RNA-sequencing analysis of activin A-treated neurons identified expression of Slc7a11 as the ferroptosis-associated gene with significant upregulation. Next, using the CRISPR/Cas9 system, mice were generated with a heterozygous deficiency of ARIP1 (Arip1^-/+), and the CNS neuroscience & therapeuticsARIP1 Deficiency Facilitates the Inhibition of Neuronal Ferroptosis in Cerebral Ischemia by Activin A Through SMAD3 and p38 MAPK Signaling.PMC124452008197117420230505042ZP81371445KYXZ2022JC04Liu HXie DZou ZLiu ZQie XZhang YGuo CWang LfalseARIP1 Deficiency Facilitates the Inhibition of Neuronal Ferroptosis in Cerebral Ischemia by Activin A Through SMAD3 and p38 MAPK Signaling.<h4>Introduction</h4>Ferroptosis is an essential pathophysiological process in cerebral ischemic injury. Activin receptor-interacting protein 1 (ARIP1) is a negative regulator of the activin signaling pathway in neurons.<h4>Objective</h4>This study investigated whether activin A inhibits neuronal ferroptosis and the role of ARIP1 in cerebral ischemic injury.<h4>Methods and results</h4>In this study, activin A increased the viability of primary neurons under conditions of oxygen-glucose deprivation (OGD). Subsequent RNA-sequencing analysis of activin A-treated neurons identified expression of Slc7a11 as the ferroptosis-associated gene with significant upregulation. Next, using the CRISPR/Cas9 system, mice were generated with a heterozygous deficiency of ARIP1 (Arip1^-/+), and the 2025-01-01T00:00:00Z2025 Sep2026-06-03T15:14:52.078Z2026-04-29T03:09:00.845ZS-EPMC124452004096596310.1111/cns.70615