{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mo X"],"funding":["National Natural Science Foundation of China","National Key Research and Development Program of China"],"pagination":["e0256024"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12445348"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["91(9)"],"pubmed_abstract":["<i>Methylorubrum extorquens</i> AM1, a native formate-utilizing bacterium, has exhibited limited capacity to tolerate formate. In this study, we employed an adaptive laboratory evolution (ALE) strategy to develop an evolved strain FT3 derived from <i>M. extorquens</i> AM1, with enhanced formate tolerance. When cultivated with a mixture of carbon sources containing 90 mM formate and 30 mM methanol, the FT3 strain exhibited 5.3 times higher optical density (OD<sub>600</sub>) compared to the parental strain. FT3 strain was shown to efficiently utilize both methanol and formate in experiments using <sup>13</sup>C-labeled carbon sources. Furthermore, the mechanism underlying the enhanced formate tolerance in FT3 strain was investigated through a combination of DNA re-sequencing, transcriptome a"],"journal":["Applied and environmental microbiology"],"pubmed_title":["Adaptively evolved &lt;i&gt;Methylorubrum extorquens&lt;/i&gt; with enhanced formate tolerance and its application in 3-hydroxypropionic acid production."],"pmcid":["PMC12445348"],"funding_grant_id":["2021YFC2103500","22478217","22078169"],"pubmed_authors":["Liu Z","Bao K","Zhu L","Ma Z","Zhang C","Yang S","Mo X","Zhao Y"],"additional_accession":[]},"is_claimable":false,"name":"Adaptively evolved &lt;i&gt;Methylorubrum extorquens&lt;/i&gt; with enhanced formate tolerance and its application in 3-hydroxypropionic acid production.","description":"<i>Methylorubrum extorquens</i> AM1, a native formate-utilizing bacterium, has exhibited limited capacity to tolerate formate. In this study, we employed an adaptive laboratory evolution (ALE) strategy to develop an evolved strain FT3 derived from <i>M. extorquens</i> AM1, with enhanced formate tolerance. When cultivated with a mixture of carbon sources containing 90 mM formate and 30 mM methanol, the FT3 strain exhibited 5.3 times higher optical density (OD<sub>600</sub>) compared to the parental strain. FT3 strain was shown to efficiently utilize both methanol and formate in experiments using <sup>13</sup>C-labeled carbon sources. Furthermore, the mechanism underlying the enhanced formate tolerance in FT3 strain was investigated through a combination of DNA re-sequencing, transcriptome a","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T14:30:39.675Z","creation":"2026-04-28T03:11:44.079Z"},"accession":"S-EPMC12445348","cross_references":{"pubmed":["40801534"],"doi":["10.1128/aem.02560-24"]}}