{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["53(17)"],"submitter":["Hengesbach M"],"funding":["Warren Alpert Foundation"],"pubmed_abstract":["The human RNome comprises all forms of RNA and the 50 + chemical structures-the epitranscriptome-that modify them. Understanding the diverse functions of RNA modifications in regulating gene expression and cell phenotype requires technologies such as RNA sequencing-based modification mapping and mass spectrometry-based quantification of modified ribonucleosides. Liquid chromatography-coupled tandem quadrupole mass spectrometry (LC-MS/MS) is the gold standard for detecting and quantifying modified ribonucleosides with accuracy and precision. However, variations in RNA isolation, processing, and LC-MS/MS analysis have hindered reproducibility across laboratories, which is essential for accurate quantification of RNA modifications. As guidance toward harmonization, we report a multi-laborator"],"journal":["Nucleic acids research"],"pagination":["gkaf895"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12445667"],"repository":["biostudies-literature"],"pubmed_title":["Toward standardized epitranscriptome analytics: an inter-laboratory comparison of mass spectrometric detection and quantification of modified ribonucleosides in human RNA."],"pmcid":["PMC12445667"],"pubmed_authors":["Chan CK","Helm M","DeMott MS","Bhandari T","Limbach PA","Dedon PC","Podoprygorina G","Sun G","Bruzel A","Tabeling E","Hengesbach M","Cheung VG"],"additional_accession":[]},"is_claimable":false,"name":"Toward standardized epitranscriptome analytics: an inter-laboratory comparison of mass spectrometric detection and quantification of modified ribonucleosides in human RNA.","description":"The human RNome comprises all forms of RNA and the 50 + chemical structures-the epitranscriptome-that modify them. Understanding the diverse functions of RNA modifications in regulating gene expression and cell phenotype requires technologies such as RNA sequencing-based modification mapping and mass spectrometry-based quantification of modified ribonucleosides. Liquid chromatography-coupled tandem quadrupole mass spectrometry (LC-MS/MS) is the gold standard for detecting and quantifying modified ribonucleosides with accuracy and precision. However, variations in RNA isolation, processing, and LC-MS/MS analysis have hindered reproducibility across laboratories, which is essential for accurate quantification of RNA modifications. As guidance toward harmonization, we report a multi-laborator","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-04-28T03:20:03.25Z","creation":"2026-04-28T03:11:29.192Z"},"accession":"S-EPMC12445667","cross_references":{"pubmed":["40966502"],"doi":["10.1093/nar/gkaf895"]}}