biostudies-literatureUnknown30Du YSteam pot chicken soup with <i>sanchi-ginseng</i> is a traditional Chinese specialty food, and its flavor formation mechanism is currently unclear. The differences of saponins, free fatty acids and small molecular metabolites in the steam pot chicken soup with sanchi-ginseng (PC), steam pot sanchi-ginseng soup (P) and steam pot chicken soup (C) groups were analyzed by HPLC, GC-MS and UHPLC-QE-MS, respectively. The results showed that the concentration of three saponins in the PC group was significantly reduced compared to the P group. The FFA concentration in PC group was significantly higher than P and C groups. 13 differential metabolites shared in common were identified in the two comparison groups. KEGG results indicated that amino acid and fatty acid metabolism were the major metaboliFood chemistry: X102933https://www.ebi.ac.uk/biostudies/studies/S-EPMC12445720biostudies-literatureUnveiling the formation mechanism of characteristic components in steam pot chicken soup with <i>sanchi-ginseng</i> based on HPLC, GC-MS combined with metabolomics.PMC12445720Yang YZhou NXun WTan JLiao GTang SDu YWang GYang CfalseUnveiling the formation mechanism of characteristic components in steam pot chicken soup with <i>sanchi-ginseng</i> based on HPLC, GC-MS combined with metabolomics.Steam pot chicken soup with <i>sanchi-ginseng</i> is a traditional Chinese specialty food, and its flavor formation mechanism is currently unclear. The differences of saponins, free fatty acids and small molecular metabolites in the steam pot chicken soup with sanchi-ginseng (PC), steam pot sanchi-ginseng soup (P) and steam pot chicken soup (C) groups were analyzed by HPLC, GC-MS and UHPLC-QE-MS, respectively. The results showed that the concentration of three saponins in the PC group was significantly reduced compared to the P group. The FFA concentration in PC group was significantly higher than P and C groups. 13 differential metabolites shared in common were identified in the two comparison groups. KEGG results indicated that amino acid and fatty acid metabolism were the major metaboli2025-01-01T00:00:00Z2025 Aug2026-06-03T17:01:53.495Z2026-05-30T03:06:21.577ZS-EPMC124457204098028710.1016/j.fochx.2025.102933