{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["74(3)"],"submitter":["Liu Y"],"pubmed_abstract":["An extremely halophilic archaeon named strain PRR65<sup>T</sup> was isolated from a salt crystal of salted seaweed knots which were purchased from a supermarket in Wuhu, China. It exhibited an ability to hydrolyze starch. Strain PRR65<sup>T</sup> is a coccus. Its growth range and optimum concentration for NaCl are 2.0-5.1 M and 3.4 M, respectively, and it requires magnesium ions (with an optimum concentration of 0.01 M). Homology search of the 16S rRNA gene sequence indicated that strain PRR65<sup>T</sup> shows the highest sequence similarities with <i>Halorubellus salinus</i> GX3<sup>T</sup> (96.97%). The basic growth conditions and many other physicochemical characteristics of strain PRR65<sup>T</sup> are distinct from those of other species within its genus. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between strain PRR65<sup>T</sup> and its close relatives were 88.97%, 86.47% and 39.2%, respectively. The DNA G + C content (mol%) for strain PRR65<sup>T</sup> is 67.2%. Based on a polyphasic taxonomic approach integrating phenotypic characteristics, chemotaxonomic markers, and comprehensive phylogenetic and genomic analyses, strain PRR65<sup>T</sup> represents a novel species within the genus <i>Halorubellus</i>. The name <i>Halorubellus amylolyticus</i> sp. nov. is proposed, with the specific epithet reflecting the organism's notable amylolytic activity. The type strain is PRR65<sup>T</sup> (= MCCC 4K00175 = KCTC 4323)."],"journal":["Polish journal of microbiology"],"pagination":["374-384"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12445982"],"repository":["biostudies-literature"],"pubmed_title":["Identification of a Novel Haloarchaeal Species &lt;i&gt;Halorubellus amylolyticus&lt;/i&gt; sp. nov., Isolated from Salt Crystals of Salted Seaweed Knots and Genomic Insights into Genus &lt;i&gt;Halorubellus&lt;/i&gt;."],"pmcid":["PMC12445982"],"pubmed_authors":["Liu Y","Shen L","Liu J","Ding Y","Lu C","Hao Y","Chen S","Jin YU","Zhang A","Shao S"],"additional_accession":[]},"is_claimable":false,"name":"Identification of a Novel Haloarchaeal Species &lt;i&gt;Halorubellus amylolyticus&lt;/i&gt; sp. nov., Isolated from Salt Crystals of Salted Seaweed Knots and Genomic Insights into Genus &lt;i&gt;Halorubellus&lt;/i&gt;.","description":"An extremely halophilic archaeon named strain PRR65<sup>T</sup> was isolated from a salt crystal of salted seaweed knots which were purchased from a supermarket in Wuhu, China. It exhibited an ability to hydrolyze starch. Strain PRR65<sup>T</sup> is a coccus. Its growth range and optimum concentration for NaCl are 2.0-5.1 M and 3.4 M, respectively, and it requires magnesium ions (with an optimum concentration of 0.01 M). Homology search of the 16S rRNA gene sequence indicated that strain PRR65<sup>T</sup> shows the highest sequence similarities with <i>Halorubellus salinus</i> GX3<sup>T</sup> (96.97%). The basic growth conditions and many other physicochemical characteristics of strain PRR65<sup>T</sup> are distinct from those of other species within its genus. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between strain PRR65<sup>T</sup> and its close relatives were 88.97%, 86.47% and 39.2%, respectively. The DNA G + C content (mol%) for strain PRR65<sup>T</sup> is 67.2%. Based on a polyphasic taxonomic approach integrating phenotypic characteristics, chemotaxonomic markers, and comprehensive phylogenetic and genomic analyses, strain PRR65<sup>T</sup> represents a novel species within the genus <i>Halorubellus</i>. The name <i>Halorubellus amylolyticus</i> sp. nov. is proposed, with the specific epithet reflecting the organism's notable amylolytic activity. The type strain is PRR65<sup>T</sup> (= MCCC 4K00175 = KCTC 4323).","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T15:39:09.589Z","creation":"2026-05-30T03:07:48.156Z"},"accession":"S-EPMC12445982","cross_references":{"pubmed":["40967815"],"doi":["10.33073/pjm-2025-032"]}}