{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6(3)"],"submitter":["Attanayake H"],"pubmed_abstract":["<h4>Objectives</h4>Efficient real-time PCR kits are commercially available for the detection of <i>Chlamydia trachomatis</i> (CT) genetic material, but at a price. As a result, cost-effective, sensitive and specific CT diagnostic tests are essential for resource limited countries. This study aims to describe the optimization of a loop mediated isothermal amplification (LAMP) assay for the detection of CT <i>ompA</i> DNA in urine.<h4>Methods</h4>Cost-saving modifications included using <i>Bsm</i> polymerase and a nucleic acid gel stain. Crude DNA extraction (method-1) involved centrifuging urine at 14,000 g for 30 min, heating the deposit at 95 °C for 5 min, and centrifuging again at 17,000 <i>g</i> for 1 min. To boost sensitivity, urinary inhibitors were diluted with phosphate-buffered sal"],"journal":["Advances in laboratory medicine"],"pagination":["314-319"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12446907"],"repository":["biostudies-literature"],"pubmed_title":["Detection of &lt;i&gt;Chlamydia trachomatis ompA&lt;/i&gt; DNA in urine by loop-mediated isothermal amplification (LAMP) assay."],"pmcid":["PMC12446907"],"pubmed_authors":["Elvitigala J","Abeygunasekera N","Goonasekara C","Gunasekera KM","Attanayake H"],"additional_accession":[]},"is_claimable":false,"name":"Detection of &lt;i&gt;Chlamydia trachomatis ompA&lt;/i&gt; DNA in urine by loop-mediated isothermal amplification (LAMP) assay.","description":"<h4>Objectives</h4>Efficient real-time PCR kits are commercially available for the detection of <i>Chlamydia trachomatis</i> (CT) genetic material, but at a price. As a result, cost-effective, sensitive and specific CT diagnostic tests are essential for resource limited countries. This study aims to describe the optimization of a loop mediated isothermal amplification (LAMP) assay for the detection of CT <i>ompA</i> DNA in urine.<h4>Methods</h4>Cost-saving modifications included using <i>Bsm</i> polymerase and a nucleic acid gel stain. Crude DNA extraction (method-1) involved centrifuging urine at 14,000 g for 30 min, heating the deposit at 95 °C for 5 min, and centrifuging again at 17,000 <i>g</i> for 1 min. To boost sensitivity, urinary inhibitors were diluted with phosphate-buffered sal","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T17:01:35.038Z","creation":"2026-05-30T03:06:15.153Z"},"accession":"S-EPMC12446907","cross_references":{"pubmed":["40977814"],"doi":["10.1515/almed-2024-0117"]}}