biostudies-literatureUnknown6(3)Attanayake H<h4>Objectives</h4>Efficient real-time PCR kits are commercially available for the detection of <i>Chlamydia trachomatis</i> (CT) genetic material, but at a price. As a result, cost-effective, sensitive and specific CT diagnostic tests are essential for resource limited countries. This study aims to describe the optimization of a loop mediated isothermal amplification (LAMP) assay for the detection of CT <i>ompA</i> DNA in urine.<h4>Methods</h4>Cost-saving modifications included using <i>Bsm</i> polymerase and a nucleic acid gel stain. Crude DNA extraction (method-1) involved centrifuging urine at 14,000 g for 30 min, heating the deposit at 95 °C for 5 min, and centrifuging again at 17,000 <i>g</i> for 1 min. To boost sensitivity, urinary inhibitors were diluted with phosphate-buffered salAdvances in laboratory medicine314-319https://www.ebi.ac.uk/biostudies/studies/S-EPMC12446907biostudies-literatureDetection of &lt;i&gt;Chlamydia trachomatis ompA&lt;/i&gt; DNA in urine by loop-mediated isothermal amplification (LAMP) assay.PMC12446907Elvitigala JAbeygunasekera NGoonasekara CGunasekera KMAttanayake HfalseDetection of &lt;i&gt;Chlamydia trachomatis ompA&lt;/i&gt; DNA in urine by loop-mediated isothermal amplification (LAMP) assay.<h4>Objectives</h4>Efficient real-time PCR kits are commercially available for the detection of <i>Chlamydia trachomatis</i> (CT) genetic material, but at a price. As a result, cost-effective, sensitive and specific CT diagnostic tests are essential for resource limited countries. This study aims to describe the optimization of a loop mediated isothermal amplification (LAMP) assay for the detection of CT <i>ompA</i> DNA in urine.<h4>Methods</h4>Cost-saving modifications included using <i>Bsm</i> polymerase and a nucleic acid gel stain. Crude DNA extraction (method-1) involved centrifuging urine at 14,000 g for 30 min, heating the deposit at 95 °C for 5 min, and centrifuging again at 17,000 <i>g</i> for 1 min. To boost sensitivity, urinary inhibitors were diluted with phosphate-buffered sal2025-01-01T00:00:00Z2025 Sep2026-06-03T17:01:35.038Z2026-05-30T03:06:15.153ZS-EPMC124469074097781410.1515/almed-2024-0117