biostudies-literatureEscude Martinez de Castilla PFondation ARC pour la Recherche sur le CancerAgence Nationale de la RechercheHorizon 2020Fondation pour la Recherche MédicaleHORIZON EUROPE Marie Sklodowska-Curie Actionse2500605https://www.ebi.ac.uk/biostudies/studies/S-EPMC12447046biostudies-literatureUnknown14(24)Prostate cancer (PCa) ranks as the fifth leading cause of cancer-related deaths among men worldwide. In 10-20% of the cases, PCa progresses to an incurable, castration-resistant stage. Castration-resistant PCa cells often overexpress prostate-specific membrane antigen (PSMA), a membrane protein that may serve as their Achilles' heel. Over the past decades, RNA-based therapeutics have emerged as promising treatments for a vast array of diseases, including cancer. In this study, with the ultimate goal of developing a targeted therapy for PCa, lipid nanoparticles (LNPs) are decorated with an anti-PSMA nanobody using click chemistry with a PEG-lipid. Direct stochastic optical reconstruction microscopy (dSTORM) and cluster analysis confirm the presence of at least one nanobody on the surface ofAdvanced healthcare materialsNanobody-Decorated Lipid Nanoparticles for Enhanced mRNA Delivery to Tumors In Vivo.PMC12447046ANR-20-CE18-0026-0ANR‐20‐CE18‐0026‐0860303825828ARCDOC42022010004513PGA1 RF20190208474AJE20160635884Escude Martinez de Castilla Pvan Niel Gvan Weerden WMvan Royen MEKooijmans SAAFens MHRietveld JSchiffelers RMJenster GVerdi Vvan Kempen SVader PKoekman ACYang QEstape Senti Mde Voogt Wvan Merris JfalseNanobody-Decorated Lipid Nanoparticles for Enhanced mRNA Delivery to Tumors In Vivo.Prostate cancer (PCa) ranks as the fifth leading cause of cancer-related deaths among men worldwide. In 10-20% of the cases, PCa progresses to an incurable, castration-resistant stage. Castration-resistant PCa cells often overexpress prostate-specific membrane antigen (PSMA), a membrane protein that may serve as their Achilles' heel. Over the past decades, RNA-based therapeutics have emerged as promising treatments for a vast array of diseases, including cancer. In this study, with the ultimate goal of developing a targeted therapy for PCa, lipid nanoparticles (LNPs) are decorated with an anti-PSMA nanobody using click chemistry with a PEG-lipid. Direct stochastic optical reconstruction microscopy (dSTORM) and cluster analysis confirm the presence of at least one nanobody on the surface of2025-01-01T00:00:00Z2025 Sep2026-06-03T14:39:08.019Z2026-05-29T03:05:30.508ZS-EPMC124470464061335210.1002/adhm.202500605