{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(9)"],"submitter":["Joubert IA"],"funding":["National Health and Medical Research Council"],"pubmed_abstract":["Dual RNA-sequencing (dual RNA-seq) holds significant promise for deciphering bacterial virulence mechanisms during systemic infections. However, its application in sepsis research is hindered by technical challenges, including a low bacterial burden in blood and limited sample volumes and RNA yield from vulnerable populations, such as neonates. We developed an optimized protocol [dual RNA isolation from blood (DRIB)] for simultaneous stabilization, isolation and purification of high-quality host leukocyte and bacterial RNA from low-volume whole blood samples (0.5 ml). This protocol is compatible with clinical sample collection workflows and high-throughput RNA sequencing. The feasibility of DRIB for dual RNA-seq was validated using a pilot cohort of clinical adult sepsis samples, enabling "],"journal":["Microbial genomics"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12447629"],"repository":["biostudies-literature"],"pubmed_title":["Dual RNA isolation from blood: an optimized protocol for host and bacterial RNA purification for dual RNA-sequencing analysis in whole blood sepsis samples."],"pmcid":["PMC12447629"],"funding_grant_id":["APP1187809","2017081"],"pubmed_authors":["Litton E","Wang P","Strunk T","Chopra A","Currie A","Raby E","Joubert IA","Mullally C"],"additional_accession":[]},"is_claimable":false,"name":"Dual RNA isolation from blood: an optimized protocol for host and bacterial RNA purification for dual RNA-sequencing analysis in whole blood sepsis samples.","description":"Dual RNA-sequencing (dual RNA-seq) holds significant promise for deciphering bacterial virulence mechanisms during systemic infections. However, its application in sepsis research is hindered by technical challenges, including a low bacterial burden in blood and limited sample volumes and RNA yield from vulnerable populations, such as neonates. We developed an optimized protocol [dual RNA isolation from blood (DRIB)] for simultaneous stabilization, isolation and purification of high-quality host leukocyte and bacterial RNA from low-volume whole blood samples (0.5 ml). This protocol is compatible with clinical sample collection workflows and high-throughput RNA sequencing. The feasibility of DRIB for dual RNA-seq was validated using a pilot cohort of clinical adult sepsis samples, enabling ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T17:29:55.269Z","creation":"2026-04-30T03:07:14.842Z"},"accession":"S-EPMC12447629","cross_references":{"pubmed":["40965975"],"doi":["10.1099/mgen.0.001501"]}}