{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Imanishi I"],"funding":["National Center for Advancing Translational Sciences","NCATS NIH HHS","American Skin Association","NCI NIH HHS","National Institutes of Health","Society for Investigative Dermatology","NIAMS NIH HHS","Regeneron Pharmaceuticals Inc","Damon Runyon Cancer Research Foundation","NIH HHS","Icahn School of Medicine at Mount Sinai"],"pagination":["116114"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12449769"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["44(8)"],"pubmed_abstract":["Chronic inflammatory skin diseases arise from dysregulated interactions between tissue-resident and infiltrating cells, the complexity of which hinders disease understanding and treatment. To address this, here, we present a single-cell spatiotemporal atlas of murine type 2 skin inflammation using MERFISH and scRNA-seq. Analyzing ∼430,000 cells during MC903- and oxazolone-induced dermatitis, we identify 39 cell types, including pro-inflammatory fibroblasts that resemble those in human atopic dermatitis. Spatial neighborhood analyses reveal basophils as potent activators of pro-inflammatory fibroblasts, with basophil-derived oncostatin-M (OSM) and IL-4 synergizing fibroblast-mediated feedforward basophil and immune recruitment. While fibroblast-specific deletion of the IL-4Rα receptor disru"],"journal":["Cell reports"],"pubmed_title":["A basophil-fibroblast pro-inflammatory axis fuels type 2 skin inflammation."],"pmcid":["PMC12449769"],"funding_grant_id":["S10OD030463","S10 OD030463","S10OD026880","S10 OD026880","K08CA263187","P30 AR079200","UL1 TR004419","T32 AR082315","T32AR082315","P30AR079200","UL1TR004419","K08 CA263187"],"pubmed_authors":["Cho I","Imanishi I","Krueger JG","Gill R","Kim BS","Wilder A","Guttman-Yassky E","Restrepo P","Nair A","Ji AL"],"additional_accession":[]},"is_claimable":false,"name":"A basophil-fibroblast pro-inflammatory axis fuels type 2 skin inflammation.","description":"Chronic inflammatory skin diseases arise from dysregulated interactions between tissue-resident and infiltrating cells, the complexity of which hinders disease understanding and treatment. To address this, here, we present a single-cell spatiotemporal atlas of murine type 2 skin inflammation using MERFISH and scRNA-seq. Analyzing ∼430,000 cells during MC903- and oxazolone-induced dermatitis, we identify 39 cell types, including pro-inflammatory fibroblasts that resemble those in human atopic dermatitis. Spatial neighborhood analyses reveal basophils as potent activators of pro-inflammatory fibroblasts, with basophil-derived oncostatin-M (OSM) and IL-4 synergizing fibroblast-mediated feedforward basophil and immune recruitment. While fibroblast-specific deletion of the IL-4Rα receptor disru","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-06-04T07:30:22.21Z","creation":"2026-06-04T03:06:05.351Z"},"accession":"S-EPMC12449769","cross_references":{"pubmed":["40782349"],"doi":["10.1016/j.celrep.2025.116114"]}}