{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rudokas V"],"funding":["Austrian Science Fund FWF","Lietuvos Mokslo Taryba","Research Collaboration Agreement between the University of Salzburg and Amsterdam Medical Center"],"pagination":["e70056"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12449780"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["102(3)"],"pubmed_abstract":["Current characterisation of cat dander/hair extracts used for allergy diagnosis or allergen-specific immunotherapy is mainly standardised towards the major allergen Fel d 1, while other allergens such as the lipocalin Fel d 7 are insufficiently characterised in such allergen products. This study aimed to produce recombinant Fel d 7 (rFel d 7) and murine IgG monoclonal antibodies (mAbs) specific to it for quantification in allergen extracts and assess the potential of the mAbs in inhibiting patients' IgE in functional assays. rFel d 7 was expressed in E. coli, purified by Ni-affinity and ion exchange chromatography, and physicochemically characterised by circular dichroism, Fourier-transform infrared spectroscopy, dynamic light scattering and mass spectrometry. Twenty hybridoma cell lines p"],"journal":["Scandinavian journal of immunology"],"pubmed_title":["Evaluation of Inhibitory Activity of Novel Monoclonal Antibodies Against Cat Allergen Fel d 7 and Their Application to Analyse Allergen Extracts."],"pmcid":["PMC12449780"],"funding_grant_id":["P34207","D‐20‐0929","D-20-0929","I5312"],"pubmed_authors":["Aglas L","Briza P","Pena-Amelunxen G","Zvirbliene A","Rudokas V"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of Inhibitory Activity of Novel Monoclonal Antibodies Against Cat Allergen Fel d 7 and Their Application to Analyse Allergen Extracts.","description":"Current characterisation of cat dander/hair extracts used for allergy diagnosis or allergen-specific immunotherapy is mainly standardised towards the major allergen Fel d 1, while other allergens such as the lipocalin Fel d 7 are insufficiently characterised in such allergen products. This study aimed to produce recombinant Fel d 7 (rFel d 7) and murine IgG monoclonal antibodies (mAbs) specific to it for quantification in allergen extracts and assess the potential of the mAbs in inhibiting patients' IgE in functional assays. rFel d 7 was expressed in E. coli, purified by Ni-affinity and ion exchange chromatography, and physicochemically characterised by circular dichroism, Fourier-transform infrared spectroscopy, dynamic light scattering and mass spectrometry. Twenty hybridoma cell lines p","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T16:57:12.579Z","creation":"2026-04-29T03:12:33.885Z"},"accession":"S-EPMC12449780","cross_references":{"pubmed":["40974251"],"doi":["10.1111/sji.70056"]}}