biostudies-literatureRudokas VAustrian Science Fund FWFLietuvos Mokslo TarybaResearch Collaboration Agreement between the University of Salzburg and Amsterdam Medical Centere70056https://www.ebi.ac.uk/biostudies/studies/S-EPMC12449780biostudies-literatureUnknown102(3)Current characterisation of cat dander/hair extracts used for allergy diagnosis or allergen-specific immunotherapy is mainly standardised towards the major allergen Fel d 1, while other allergens such as the lipocalin Fel d 7 are insufficiently characterised in such allergen products. This study aimed to produce recombinant Fel d 7 (rFel d 7) and murine IgG monoclonal antibodies (mAbs) specific to it for quantification in allergen extracts and assess the potential of the mAbs in inhibiting patients' IgE in functional assays. rFel d 7 was expressed in E. coli, purified by Ni-affinity and ion exchange chromatography, and physicochemically characterised by circular dichroism, Fourier-transform infrared spectroscopy, dynamic light scattering and mass spectrometry. Twenty hybridoma cell lines pScandinavian journal of immunologyEvaluation of Inhibitory Activity of Novel Monoclonal Antibodies Against Cat Allergen Fel d 7 and Their Application to Analyse Allergen Extracts.PMC12449780P34207D‐20‐0929D-20-0929I5312Aglas LBriza PPena-Amelunxen GZvirbliene ARudokas VfalseEvaluation of Inhibitory Activity of Novel Monoclonal Antibodies Against Cat Allergen Fel d 7 and Their Application to Analyse Allergen Extracts.Current characterisation of cat dander/hair extracts used for allergy diagnosis or allergen-specific immunotherapy is mainly standardised towards the major allergen Fel d 1, while other allergens such as the lipocalin Fel d 7 are insufficiently characterised in such allergen products. This study aimed to produce recombinant Fel d 7 (rFel d 7) and murine IgG monoclonal antibodies (mAbs) specific to it for quantification in allergen extracts and assess the potential of the mAbs in inhibiting patients' IgE in functional assays. rFel d 7 was expressed in E. coli, purified by Ni-affinity and ion exchange chromatography, and physicochemically characterised by circular dichroism, Fourier-transform infrared spectroscopy, dynamic light scattering and mass spectrometry. Twenty hybridoma cell lines p2025-01-01T00:00:00Z2025 Sep2026-06-03T16:57:12.579Z2026-04-29T03:12:33.885ZS-EPMC124497804097425110.1111/sji.70056