biostudies-literatureUnknown16Yang F<h4>Introduction</h4><i>Tetrastigma hemsleyanum</i> Diels et Gilg (Vitaceae) is a medicinally valuable plant typically with a whitish root cross-section. Resource surveys discovered some roots of <i>T. hemsleyanum</i> with a yellow-brown root cross-section and stronger antioxidant properties. However, the molecular mechanisms underlying these phenotypic differences remain poorly understood.<h4>Methods</h4>We employed integrated metabolomic and transcriptomic analyses to compare the two root types. Key candidate genes were cloned and functionally characterized to uncover the regulatory mechanisms involved.<h4>Results</h4>Metabolomic analysis identified significant changes in 217 metabolites between the two types, among which 57 flavonoids, such as Hesperetin-7-O-glucoside, were significantlFrontiers in plant science1642835https://www.ebi.ac.uk/biostudies/studies/S-EPMC12450873biostudies-literatureMulti-omics analysis reveals ThMYB6 regulation of flavonoid biosynthesis in differently colored tuberous roots of &lt;i&gt;Tetrastigma hemsleyanum&lt;/i&gt;.PMC12450873Shen QLi SGu YLiu SWang HFeng BBai YWang ZYang FXing QfalseMulti-omics analysis reveals ThMYB6 regulation of flavonoid biosynthesis in differently colored tuberous roots of &lt;i&gt;Tetrastigma hemsleyanum&lt;/i&gt;.<h4>Introduction</h4><i>Tetrastigma hemsleyanum</i> Diels et Gilg (Vitaceae) is a medicinally valuable plant typically with a whitish root cross-section. Resource surveys discovered some roots of <i>T. hemsleyanum</i> with a yellow-brown root cross-section and stronger antioxidant properties. However, the molecular mechanisms underlying these phenotypic differences remain poorly understood.<h4>Methods</h4>We employed integrated metabolomic and transcriptomic analyses to compare the two root types. Key candidate genes were cloned and functionally characterized to uncover the regulatory mechanisms involved.<h4>Results</h4>Metabolomic analysis identified significant changes in 217 metabolites between the two types, among which 57 flavonoids, such as Hesperetin-7-O-glucoside, were significantl2025-01-01T00:00:00Z20252026-04-30T03:19:59.562Z2026-04-30T03:12:03.506ZS-EPMC124508734098973010.3389/fpls.2025.1642835