biostudies-literatureUnknown16Du M<h4>Introduction</h4>The RWP-RK transcription factor family plays a pivotal role in nitrogen response and gametophyte development. Despite its biological importance, the evolutionary relationships and functional characteristics of RWP-RK genes in rice remain incompletely understood. This study aimed to investigate the structure, classification, expression patterns, and potential regulatory roles of RWP-RK transcription factors in rice (Oryza sativa), with a focus on their involvement in nitrogen signaling and reproductive development.<h4>Methods</h4>A comprehensive genome-wide analysis was conducted to identify RWP-RK genes in rice. A total of 13 genes encoding 14 proteins, including two alternative splicing variants of OsNLP5, were identified and mapped across 8 of the 12 rice chromosomesFrontiers in plant science1597029https://www.ebi.ac.uk/biostudies/studies/S-EPMC12450915biostudies-literatureGenome-wide analysis of RWP-RK transcription factor family reveals its roles in nitrogen response in rice (<i>Oryza sativa</i>).PMC12450915Xu LGong SGuo ZDu MfalseGenome-wide analysis of RWP-RK transcription factor family reveals its roles in nitrogen response in rice (<i>Oryza sativa</i>).<h4>Introduction</h4>The RWP-RK transcription factor family plays a pivotal role in nitrogen response and gametophyte development. Despite its biological importance, the evolutionary relationships and functional characteristics of RWP-RK genes in rice remain incompletely understood. This study aimed to investigate the structure, classification, expression patterns, and potential regulatory roles of RWP-RK transcription factors in rice (Oryza sativa), with a focus on their involvement in nitrogen signaling and reproductive development.<h4>Methods</h4>A comprehensive genome-wide analysis was conducted to identify RWP-RK genes in rice. A total of 13 genes encoding 14 proteins, including two alternative splicing variants of OsNLP5, were identified and mapped across 8 of the 12 rice chromosomes2025-01-01T00:00:00Z20252026-04-30T03:21:52.797Z2026-04-30T03:11:29.266ZS-EPMC124509154098973110.3389/fpls.2025.1597029