{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hu X"],"funding":["American Diabetes Association","JDRF"],"pagination":["1825-1838"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12451085"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["74(10)"],"pubmed_abstract":["Metabolic stress elicits functional changes in pancreatic islets, contributing to the pathogenesis of type 2 diabetes. However, the molecular mechanisms underlying overnutrition stress in islet cells is not well understood. In our study, we subjected human islets to overnutrition with 25 mmol/L glucose and 0.5 mmol/L palmitic acid (glucolipotoxicity) or to a control culture condition with 5.1 mmol/L glucose. We used single-cell RNA sequencing to comprehensively characterize the gene expression changes between these two conditions in a cell type-specific manner. We found that among all islet endocrine cell types, α-cells were the most resilient to glucolipotoxicity, while β-cells were the most susceptible. We also observed a reduction in cell-cell interactions within islet endocrine cells u"],"journal":["Diabetes"],"pubmed_title":["Molecular Mechanisms of Human Pancreatic Islet Dysfunction Under Overnutrition Metabolic Stress."],"pmcid":["PMC12451085"],"funding_grant_id":["1-INO-2022-1129-A-N","11-22-JDFPM-03"],"pubmed_authors":["Pilar Toledo M","Irianto J","Naji A","Guo L","Xie G","Hu X","Liu C","Roper MG","Sandoval Sanchez P","Wang YJ"],"additional_accession":[]},"is_claimable":false,"name":"Molecular Mechanisms of Human Pancreatic Islet Dysfunction Under Overnutrition Metabolic Stress.","description":"Metabolic stress elicits functional changes in pancreatic islets, contributing to the pathogenesis of type 2 diabetes. However, the molecular mechanisms underlying overnutrition stress in islet cells is not well understood. In our study, we subjected human islets to overnutrition with 25 mmol/L glucose and 0.5 mmol/L palmitic acid (glucolipotoxicity) or to a control culture condition with 5.1 mmol/L glucose. We used single-cell RNA sequencing to comprehensively characterize the gene expression changes between these two conditions in a cell type-specific manner. We found that among all islet endocrine cell types, α-cells were the most resilient to glucolipotoxicity, while β-cells were the most susceptible. We also observed a reduction in cell-cell interactions within islet endocrine cells u","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-03T03:09:48.362Z","creation":"2026-06-03T03:07:30.484Z"},"accession":"S-EPMC12451085","cross_references":{"pubmed":["40773375"],"doi":["10.2337/db24-1038"]}}