{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(3)"],"submitter":["McComb S"],"pubmed_abstract":["<h4>Background</h4>Production of chimeric antigen receptor T cell (CAR-T) therapies depends on antibody reagents to label, isolate, and expand T cell products. We sought to create antibody tools specific for the variable domain of heavy-chain only antibodies (VHHs), also known as nanobodies, used in some CARs.<h4>Methods</h4>We immunized a mouse with VHH and selected two murine monoclonal antibodies (mAbs) that bind to distinct epitopes in conserved framework regions of llama-derived VHHs, and not to human VH domains. Anti-VHH mAbs were characterized by enzyme-linked immunosorbent assay, surface plasmon resonance, and hydrogen-deuterium exchange mass spectrometry; were then tested for cell/tissue labeling and for modulating cellular activity in VHH-CAR-T cells.<h4>Results</h4>We produced a"],"journal":["Antibody therapeutics"],"pagination":["242-258"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12451263"],"repository":["biostudies-literature"],"pubmed_title":["Broadly reactive anti-VHH antibodies for characterizing, blocking, or activating nanobody-based CAR-T cells."],"pmcid":["PMC12451263"],"pubmed_authors":["Aubry A","Acchione M","Coutu M","Acel A","Wu C","Marcil A","Joubert S","Tremblay TL","Moraitis AN","Parat M","Hill JJ","Zafer A","Weeratna RD","Malenfant F","Pon RA","Pohankova P","Page M","Shepherd A","Hussack G","El Bakkouri M","Lippens J","Raphael S","Plante P","Bennychen B","Sheff J","Lamoureux L","Nguyen T","Arbabi-Ghahroudi M","Webb J","Smith E","McComb S","Dupont B","Fortin A","Gadoury C","Tamblyn L","Faulkes S","Manceur AP","Schrag J","Zhu Q"],"additional_accession":[]},"is_claimable":false,"name":"Broadly reactive anti-VHH antibodies for characterizing, blocking, or activating nanobody-based CAR-T cells.","description":"<h4>Background</h4>Production of chimeric antigen receptor T cell (CAR-T) therapies depends on antibody reagents to label, isolate, and expand T cell products. We sought to create antibody tools specific for the variable domain of heavy-chain only antibodies (VHHs), also known as nanobodies, used in some CARs.<h4>Methods</h4>We immunized a mouse with VHH and selected two murine monoclonal antibodies (mAbs) that bind to distinct epitopes in conserved framework regions of llama-derived VHHs, and not to human VH domains. Anti-VHH mAbs were characterized by enzyme-linked immunosorbent assay, surface plasmon resonance, and hydrogen-deuterium exchange mass spectrometry; were then tested for cell/tissue labeling and for modulating cellular activity in VHH-CAR-T cells.<h4>Results</h4>We produced a","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-06-03T19:19:11.379Z","creation":"2026-05-30T03:06:49.475Z"},"accession":"S-EPMC12451263","cross_references":{"pubmed":["40989106"],"doi":["10.1093/abt/tbaf011"]}}