{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(41)"],"submitter":["Jedinakova P"],"pubmed_abstract":["A new methodology for the detection of three model proteases using a multi-purpose peptide probe equipped with three selectively cleavable sites and four fluorophores was developed and studied. The probe was designed as a single-excitation, triple-emission system, allowing for the monitoring of characteristic real-time changes in the fluorescence emission responses of individual fluorophores during enzymatic cleavage. It was labelled with diethylaminocoumarin (DEAC), fluorescein (FL) and Rhodamine B (RhB), forming the DEAC → FL → RhB FRET-cascade, with estimated Förster distances of 3.08 ± 0.02 nm or 3.02 ± 0.02 nm for the DEAC → FL FRET-pair (depending on the DEAC labeling site) and 6.04 ± 0.05 nm for the FL → RhB FRET-pair. Although spectroscopic analyses indicate that photophysical proc"],"journal":["RSC advances"],"pagination":["34821-34832"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12451675"],"repository":["biostudies-literature"],"pubmed_title":["A new multi-purpose FRET fluorescent probe for the simultaneous detection of proteases."],"pmcid":["PMC12451675"],"pubmed_authors":["Barbuscakova Z","Milicevic D","Hlavac J","Kubala M","Jedinakova P"],"additional_accession":[]},"is_claimable":false,"name":"A new multi-purpose FRET fluorescent probe for the simultaneous detection of proteases.","description":"A new methodology for the detection of three model proteases using a multi-purpose peptide probe equipped with three selectively cleavable sites and four fluorophores was developed and studied. The probe was designed as a single-excitation, triple-emission system, allowing for the monitoring of characteristic real-time changes in the fluorescence emission responses of individual fluorophores during enzymatic cleavage. It was labelled with diethylaminocoumarin (DEAC), fluorescein (FL) and Rhodamine B (RhB), forming the DEAC → FL → RhB FRET-cascade, with estimated Förster distances of 3.08 ± 0.02 nm or 3.02 ± 0.02 nm for the DEAC → FL FRET-pair (depending on the DEAC labeling site) and 6.04 ± 0.05 nm for the FL → RhB FRET-pair. Although spectroscopic analyses indicate that photophysical proc","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T19:18:41.364Z","creation":"2026-05-30T03:06:36.992Z"},"accession":"S-EPMC12451675","cross_references":{"pubmed":["40989513"],"doi":["10.1039/d5ra03163j"]}}