{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mayle R"],"funding":["Foundation for the NIH (FNIH)","NIGMS NIH HHS"],"pagination":["e2517556122"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12452832"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["122(37)"],"pubmed_abstract":["Replication of cellular chromosomes requires a primase to generate short RNA primers to initiate genomic replication. While bacterial and archaeal primase generate short RNA primers, the eukaryotic primase, Polα-primase, contains both RNA primase and DNA polymerase (Pol) subunits that function together to form a >20 base hybrid RNA-DNA primer. Interestingly, the DNA Pol1 subunit of Polα lacks a 3'-5' proofreading exonuclease, contrary to the high-fidelity normally associated with DNA replication. However, Polδ and Polε synthesize the majority of the eukaryotic genome, and both contain 3'-5' exonuclease activity for high fidelity. Nonetheless, even the small amount of DNA produced by Pol1 in each of the many RNA/DNA primers during chromosome replication adds up to tens of millions of nucleo"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["DNA polymerase α-primase can function as a translesion DNA polymerase."],"pmcid":["PMC12452832"],"funding_grant_id":["R35 GM148159","GM149862","R01 GM149862"],"pubmed_authors":["Mayle R","O'Donnell ME","Georgescu R"],"additional_accession":[]},"is_claimable":false,"name":"DNA polymerase α-primase can function as a translesion DNA polymerase.","description":"Replication of cellular chromosomes requires a primase to generate short RNA primers to initiate genomic replication. While bacterial and archaeal primase generate short RNA primers, the eukaryotic primase, Polα-primase, contains both RNA primase and DNA polymerase (Pol) subunits that function together to form a >20 base hybrid RNA-DNA primer. Interestingly, the DNA Pol1 subunit of Polα lacks a 3'-5' proofreading exonuclease, contrary to the high-fidelity normally associated with DNA replication. However, Polδ and Polε synthesize the majority of the eukaryotic genome, and both contain 3'-5' exonuclease activity for high fidelity. Nonetheless, even the small amount of DNA produced by Pol1 in each of the many RNA/DNA primers during chromosome replication adds up to tens of millions of nucleo","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T19:13:17.688Z","creation":"2026-05-30T03:06:33.397Z"},"accession":"S-EPMC12452832","cross_references":{"pubmed":["40928879"],"doi":["10.1073/pnas.2517556122"]}}