biostudies-literatureRuijter NH2020 Industrial Leadership4105-4130https://www.ebi.ac.uk/biostudies/studies/S-EPMC12454472biostudies-literatureUnknown99(10)There is an increasing need for new approach methodologies (NAMs) for safety assessment of nanomaterials (NMs) in order to keep pace with innovation. In vitro assays are useful tools during pre-market hazard screening approaches of NMs to prioritize safe(r) candidate NMs and reduce the amount of regulatory testing required. For pre-regulatory hazard screening applications, it is crucial that in vitro assays have the capacity to distinguish between NMs based on their hazard potency and have the ability to provide accurate hazard rankings. In this paper, four types of silica particles (crystalline, pyrogenic, colloidal, and silane functionalized colloidal) were subjected to twenty-four in vitro assays to obtain hazard rankings using dose-response modelling. The assays were chosen for their rArchives of toxicologyApplication of in vitro pulmonary models for hazard screening of silica particles.PMC12454472862419Vermeulen JMarshall JBraakhuis HKatsumiti ABoyles MGarriz IPZanoni IRuijter NCassee FRCarriere MCandalija AfalseApplication of in vitro pulmonary models for hazard screening of silica particles.There is an increasing need for new approach methodologies (NAMs) for safety assessment of nanomaterials (NMs) in order to keep pace with innovation. In vitro assays are useful tools during pre-market hazard screening approaches of NMs to prioritize safe(r) candidate NMs and reduce the amount of regulatory testing required. For pre-regulatory hazard screening applications, it is crucial that in vitro assays have the capacity to distinguish between NMs based on their hazard potency and have the ability to provide accurate hazard rankings. In this paper, four types of silica particles (crystalline, pyrogenic, colloidal, and silane functionalized colloidal) were subjected to twenty-four in vitro assays to obtain hazard rankings using dose-response modelling. The assays were chosen for their r2025-01-01T00:00:00Z2025 Oct2026-06-03T18:51:32.716Z2026-04-30T03:11:28.709ZS-EPMC124544724056019210.1007/s00204-025-04100-5